Kitayama Shuzo, Wong Lok O, Ma Li, Hao Jia, Kasugai Shohei, Lang Niklaus P, Mattheos Nikos
Implant Dentistry, Faculty of Dentistry, The University of Hong Kong, Hong Kong.
Oral Implantology and Regenerative Dental Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
Clin Oral Implants Res. 2016 Dec;27(12):e206-e214. doi: 10.1111/clr.12605. Epub 2015 Apr 27.
Biphasic calcium phosphate (BCP) composed of 10% hydroxyapatite (HA) and 90% beta-tricalcium phosphate has been developed. Recently, a strontium hydroxyapatite-containing collagen membrane (Sr) was shown to stimulate early bone formation in rat calvarial defects at 4 weeks postoperatively, as compared with a cross-linked collagen membrane, for guided bone regeneration (GBR). The objective of this study was to evaluate these novel biomaterials for GBR in relation to a non-cross-linked collagen membrane (BG) and deproteinized bovine bone mineral (BO).
Twenty New Zealand rabbits were used in this study. Four defects of 7 mm in diameter were created in each rabbit, and three of the defects were treated with BG/BO, Sr/BO, and Sr/BCP. Ten rabbits were sacrificed at 12 and 24 weeks, respectively. Histological and histomorphometric analyses were conducted. Volumetric densities of mineralized new bone (MNB), bone marrow (BM), residual grafting material (RG), and non-mineralized connective tissue (NCT) were determined for each group.
After 12 weeks, Sr/BCP yielded more MNB than BG/BO and Sr/BO with no significant difference among the three groups. After 24 weeks, however, Sr/BCP demonstrated significantly more MNB than BG/BO and Sr/BO. Both after 12 and 24 weeks, Sr/BCP showed significantly less RG than BG/BO and Sr/BO. There was a significant increase in MNB in Sr/BCP from 12 to 24 weeks.
In defects grafted with BCP, more MNB was formed while less RG remained, than in defects grafted with BO. The Sr membrane was as effective as BG when comparing Sr/BO with BG/BO.
已研发出由10%羟基磷灰石（HA）和90%β-磷酸三钙组成的双相磷酸钙（BCP）。最近，与交联胶原膜相比，含锶羟基磷灰石的胶原膜（Sr）在大鼠颅骨缺损术后4周时可刺激早期骨形成，用于引导骨再生（GBR）。本研究的目的是评估这些新型生物材料与非交联胶原膜（BG）和脱蛋白牛骨矿物质（BO）相比在GBR中的应用。
本研究使用了20只新西兰兔。每只兔制造4个直径7毫米的缺损，其中3个缺损分别用BG/BO、Sr/BO和Sr/BCP处理。分别在12周和24周时处死10只兔。进行组织学和组织形态计量学分析。测定每组矿化新骨（MNB）、骨髓（BM）、残余移植材料（RG）和非矿化结缔组织（NCT）的体积密度。
12周后，Sr/BCP产生的MNB比BG/BO和Sr/BO更多，三组之间无显著差异。然而，24周后，Sr/BCP显示出的MNB明显多于BG/BO和Sr/BO。在12周和24周后，Sr/BCP显示出的RG均明显少于BG/BO和Sr/BO。从12周到24周，Sr/BCP中的MNB有显著增加。
与用BO移植的缺损相比，在用BCP移植的缺损中形成了更多的MNB，同时剩余的RG更少。当比较Sr/BO与BG/BO时，Sr膜与BG一样有效。
