Atta Diaa, Okasha Ali
Spectroscopy Department, Physics Division, National Research Center, 12311 Dokki, Cairo, Egypt.
Spectroscopy Department, Physics Division, National Research Center, 12311 Dokki, Cairo, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Jan 25;135:1173-9. doi: 10.1016/j.saa.2014.07.085. Epub 2014 Aug 8.
In this article, we discussed some single molecule spectroscopy techniques and methods. We have chosen the simplicity in this survey based on our laboratory experience in this field. We concentrated on the imaging by both techniques the wide field and the scanning microscopes. Other imaging enhancements on the technique like extended resolution wide field, the total internal reflection imaging, and its derivatives are also reviewed. In addition to the imaging techniques, some diffusion techniques also are discussed like fluorescence correlation spectroscopy. The related methods like Forester resonance transfer, photo-induced electron transfer and anisotropy (steady state and time decay) are also discussed. In addition, we elucidated some simple details about the theory behind the FCS and its resulting curve fitting. This review is preceded by general introduction and ended with the conclusion.
在本文中，我们讨论了一些单分子光谱技术和方法。基于我们在该领域的实验室经验，我们在本次综述中选择了简洁性。我们专注于通过宽场和扫描显微镜这两种技术进行成像。还对该技术的其他成像增强方法进行了综述，如扩展分辨率宽场、全内反射成像及其衍生技术。除了成像技术外，还讨论了一些扩散技术，如荧光相关光谱法。还讨论了诸如福斯特共振转移、光诱导电子转移和各向异性（稳态和时间衰减）等相关方法。此外，我们阐明了关于荧光相关光谱法背后的理论及其所得曲线拟合的一些简单细节。本综述以一般性介绍开篇，以结论收尾。
