Zheng H L, Li H, Sun Y S, Yang Z Y, Yu Q
Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest Agriculture and Forestry University, Yangling, Shaanxi Province, China
Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest Agriculture and Forestry University, Yangling, Shaanxi Province, China.
Genet Mol Res. 2014 Aug 25;13(3):6448-54. doi: 10.4238/2014.August.25.8.
Parathyroid hormone-related peptide (PTHrP) plays important roles in promoting cancer occurrence and in the development of bone metastases. To increase our knowledge of the biological functions of PTHrP, the prokaryotic expression vector pET-PTHrP was successfully constructed and the His-PTHrP fusion protein was expressed in Escherichia coli. Anti-PTHrP polyclonal antibody was then prepared from rabbits. Finally, the goat tissue expression profile of PTHrP was analyzed by Western blot with the anti-PTHrP polyclonal antibody. The results showed that the expression of PTHrP in goat mammary glands was significantly higher than that in other organs. This indicates that PTHrP may play important roles in the goat mammary gland. The antibody prepared will be a useful tool for detecting PTHrP and will be valuable in future studies investigating the role of PTHrP in calcium metabolism in the goat model.
甲状旁腺激素相关肽（PTHrP）在促进癌症发生及骨转移发展过程中发挥着重要作用。为增进我们对PTHrP生物学功能的了解，成功构建了原核表达载体pET - PTHrP，并在大肠杆菌中表达了His - PTHrP融合蛋白。随后用兔子制备了抗PTHrP多克隆抗体。最后，用抗PTHrP多克隆抗体通过蛋白质免疫印迹法分析了山羊组织中PTHrP的表达谱。结果显示，PTHrP在山羊乳腺中的表达显著高于其他器官。这表明PTHrP可能在山羊乳腺中发挥重要作用。所制备的抗体将成为检测PTHrP的有用工具，并且在未来研究PTHrP在山羊模型钙代谢中的作用时具有重要价值。
