Callaini G, Dallai R, Riparbelli M G
Department of Evolutionary Biology, University of Siena, Italy.
Biol Cell. 1989;67(3):313-20.
Drosophila embryos, because of their high proportion of dividing nuclei, offer many advantages for the study of the mitotic cycle. In the present study we combined immunofluorescence with interference contrast techniques to follow centrosome and spindle behavior in embryos exposed to diazepam during the first stages of development. Exposure to 100 micrograms/ml of diazepam produced polyploid and aneuploid figures resulting from the unusual fusion of one or more adjacent spindles. Diazepam also causes the inhibition of centrosome shifting and induces the formation of monopolar spindles during the metaphase-anaphase transition.
果蝇胚胎由于其分裂细胞核的比例很高，为研究有丝分裂周期提供了许多优势。在本研究中，我们将免疫荧光与干涉对比技术相结合，以追踪发育早期暴露于地西泮的胚胎中的中心体和纺锤体行为。暴露于100微克/毫升的地西泮会导致多倍体和非整倍体形态，这是由一个或多个相邻纺锤体的异常融合引起的。地西泮还会导致中心体移位受到抑制，并在中期 - 后期转变期间诱导单极纺锤体的形成。
