Freeman M, Nüsslein-Volhard C, Glover D M
Cell. 1986 Aug 1;46(3):457-68. doi: 10.1016/0092-8674(86)90666-5.
We describe a recessive, maternal-effect lethal mutation of Drosophila, gnu. gnu uncouples nuclear division from many cytoplasmic events of mitosis in the Drosophila embryo. Embryos from homozygous females are defective in nuclear division, but not in DNA replication, and therefore develop a small number of giant nuclei. Centrosomes divide independently of nuclear division and migrate to the surface of the syncytial blastoderm. There they nucleate microtubules into asters, which appear normal at first but become very large. Only later, when the giant nuclei begin to break down, are spindles sometimes formed. The cortical actin of these embryos develops into a characteristic network.
我们描述了一种果蝇的隐性母性效应致死突变体gnu。gnu使果蝇胚胎中的核分裂与许多有丝分裂的细胞质事件解偶联。来自纯合雌性的胚胎在核分裂方面存在缺陷，但在DNA复制方面没有缺陷，因此会发育出少量的巨型细胞核。中心体独立于核分裂进行分裂，并迁移到合胞体胚盘的表面。在那里，它们将微管聚集成星体，这些星体起初看起来正常，但后来会变得非常大。只有在后期，当巨型细胞核开始分解时，有时才会形成纺锤体。这些胚胎的皮质肌动蛋白发育成一种特征性网络。
