Eldrup E, Christensen N J, Andreasen J, Hilsted J
Department of Internal Medicine and Endocrinology, Herlev University Hospital, Denmark.
Eur J Clin Invest. 1989 Dec;19(6):514-7. doi: 10.1111/j.1365-2362.1989.tb00268.x.
To clarify the origin of plasma DOPA (3,4-Dihydroxyphenylalanine), the relationship between plasma DOPA and acute or chronic changes in sympathetic activity has been studied. Plasma DOPA and noradrenaline (NA) concentrations were measured by reverse-phase high-performance liquid chromatography with electrochemical detection. Administration of clonidine to healthy men decreased plasma NE markedly compared to no drug. Plasma DOPA decreased slightly but significantly with time, but values were identical after clonidine compared to no drug. Baseline plasma NE concentrations were significantly reduced in diabetic patients with autonomic neuropathy compared to diabetics without neuropathy, whereas baseline plasma DOPA concentrations were similar in the three groups investigated: 6.55 (5.03-7.26, median [interquartile range], n = 8) nmol l-1 in diabetics with neuropathy, 7.41 (5.79-7.97, n = 8) nmol l-1 in diabetics without neuropathy, and 6.85 (5.58-7.36, n = 8) nmol l-1 in controls. No relationship was obtained between baseline values of plasma NE and plasma DOPA. Plasma DOPA did not change in the upright position, whereas plasma NE increased significantly. Our results indicate that plasma DOPA is not related to sympathetic activity and may be of non-neuronal origin.
为阐明血浆多巴胺（3,4-二羟基苯丙氨酸）的来源，研究了血浆多巴胺与交感神经活动急性或慢性变化之间的关系。采用反相高效液相色谱-电化学检测法测定血浆多巴胺和去甲肾上腺素（NA）浓度。与未用药相比，给健康男性服用可乐定后血浆去甲肾上腺素显著降低。血浆多巴胺随时间略有但显著下降，但可乐定组与未用药组相比，其值相同。与无神经病变的糖尿病患者相比，自主神经病变的糖尿病患者基线血浆去甲肾上腺素浓度显著降低，而在研究的三组中，基线血浆多巴胺浓度相似：神经病变糖尿病患者为6.55（5.03 - 7.26，中位数[四分位间距]，n = 8）nmol/L，无神经病变糖尿病患者为7.41（5.79 - 7.97，n = 8）nmol/L，对照组为6.85（5.58 - 7.36，n = 8）nmol/L。血浆去甲肾上腺素和血浆多巴胺的基线值之间未发现相关性。血浆多巴胺在直立位时无变化，而血浆去甲肾上腺素显著增加。我们的结果表明，血浆多巴胺与交感神经活动无关，可能来源于非神经元。
