Hengjiu Tian, Jianwei Ji, Shi Yang, Zhiming Zhang, Laghari Muhammad Younis, Narejo Naeem Tariq, Lashari Punhal
a Beijing Wildlife Rescue and Rehabilitation Center , Beijing , P.R. China .
b School of Economics and Management, Beijing Forestry University , Beijing , P.R. China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):1455-6. doi: 10.3109/19401736.2014.953090. Epub 2014 Aug 27.
In the present study, the complete mitochondrial genome sequence of Bubo bubo using PCR amplification, sequencing and assembling has been obtained for the first time. The total length of the mitochondrial genome was 16,250 bp, with the base composition of 29.88% A, 34.16% C, 14.35% G, and 21.58% T. It contained 37 genes (2 ribosomal RNA genes, 13 protein-coding genes and 22 transfer RNA genes) and a major non-coding control region (D-loop region). The complete mitochondrial genome sequence of Bubo bubo provides an important data set for further investigation on the phylogenetic relationships within Strigiformes.
在本研究中,首次通过聚合酶链反应(PCR)扩增、测序和组装获得了雕鸮的完整线粒体基因组序列。线粒体基因组全长16250bp,碱基组成为:腺嘌呤(A)29.88%、胞嘧啶(C)34.16%、鸟嘌呤(G)14.35%、胸腺嘧啶(T)21.58%。它包含37个基因(2个核糖体RNA基因、13个蛋白质编码基因和22个转运RNA基因)以及一个主要的非编码控制区(D环区)。雕鸮的完整线粒体基因组序列为进一步研究鸮形目内部的系统发育关系提供了重要的数据集。