College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.
College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.
Int J Biol Macromol. 2020 May 15;151:924-931. doi: 10.1016/j.ijbiomac.2020.02.238. Epub 2020 Feb 22.
New advances in molecular approaches for DNA analysis have enhanced our understanding of the phylogenetic relationship of birds. The Little Owl (Athene noctua) is of great significance for the integrated management of forest diseases and control of regional pests. Here, we sequenced and annotated the 17,772 bp complete mitogenome of A. noctua. The mitogenome encoded 37 typical mitochondrial genes: 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA, and one non-coding control region (D-loop). The organization and location of genes in the A. noctua mitogenome were consistent with those reported for other Strigidae birds. Phylogenetic relationships based on Bayesian inference and Maximum likelihood methods showed that A. noctua has close relationships with Athene brama and Glaucidium cuculoides, confirming that A. noctua belongs to the Strigidae family. The phylogenetic relationships among seven genera of the Strigidae family used in this study were: Ninox and the other six genera were far apart, Otus and the clade ((Bubo + Strix) + Asio) were clustered into one branch, and Athene and Glaucidium were clustered into one branch. This phylogenetic classification is consistent with prior taxonomic studies on the Strigidae family. Our results provide new mitogenomic data to support further phylogenetic and taxonomic studies of Strigidae.
分子方法在 DNA 分析方面的新进展增强了我们对鸟类系统发育关系的理解。小鸮（Athene noctua）对于森林病虫害的综合治理和区域性害虫的防治具有重要意义。本研究对 A. noctua 的 17772bp 完整线粒体基因组进行了测序和注释。线粒体基因组编码了 37 个典型的线粒体基因：13 个蛋白编码基因、两个核糖体 RNA 基因、22 个转移 RNA 和一个非编码控制区（D 环）。A. noctua 线粒体基因组中基因的组织和位置与其他 Strigidae 鸟类的报告一致。基于贝叶斯推断和最大似然法的系统发育关系显示，A. noctua 与 Athene brama 和 Glaucidium cuculoides 关系密切，证实 A. noctua 属于 Strigidae 科。本研究中使用的 Strigidae 科七个属的系统发育关系为：Ninox 与其他六个属相距较远，Otus 与（Bubo + Strix）+ Asio 聚类为一个分支，Athene 和 Glaucidium 聚类为一个分支。这种系统发育分类与 Strigidae 科的先前分类学研究一致。本研究结果为进一步研究 Strigidae 的系统发育和分类学提供了新的线粒体基因组数据支持。
