Watanabe Y, Gyotoku J, Katsura Y
Department of Immunology, Chest Disease Research Institute, Kyoto University, Japan.
Thymus. 1989;13(1-2):57-71.
Development of T cells from precursors in the bone marrow or fetal thymus cells was investigated by transferring these cells into deoxyguanosine (dGuo)-treated organ cultured fetal thymus (FTOC) with a microinjector (micro i.t. method). Donor type T cells could be generated after transfer by the micro i.t. method of precursors present both in bone marrow and fetal thymus. The kinetics of the generation of T cells from these precursors was similar to that found in in vivo intra thymus transfer systems. As low as 300 fetal thymus cells were shown to be sufficient to repopulate a dGuo-FTOC. On the other hand, in a hanging drop culture method only precursors from the fetal thymus but not from the bone marrow were effective in repopulating the lobes. The hanging drop method was ineffective in repopulating all lobes, even when higher numbers of fetal thymus cells were applied. Moreover the number of T cells recovered per repopulated lobes was 2 to 3 times lower in the hanging drop system than in the micro i.t. system. It was also observed that the development of T cells was significantly accelerated by adding adherent cells from either the thymus or the spleen.
通过使用微量注射器将骨髓前体细胞或胎儿胸腺细胞转移至经脱氧鸟苷（dGuo）处理的器官培养胎儿胸腺（FTOC）中（微量注射法），对T细胞从这些前体细胞发育而来的情况进行了研究。通过微量注射法转移骨髓和胎儿胸腺中存在的前体细胞后，可产生供体类型的T细胞。这些前体细胞产生T细胞的动力学与体内胸腺内转移系统中的情况相似。研究表明，低至300个胎儿胸腺细胞就足以使dGuo - FTOC重新填充细胞。另一方面，在悬滴培养法中，只有来自胎儿胸腺而非骨髓的前体细胞能够有效地使叶重新填充细胞。即使应用了更多数量的胎儿胸腺细胞，悬滴法也无法有效地使所有叶重新填充细胞。此外，在悬滴系统中，每个重新填充叶回收的T细胞数量比微量注射系统低2至3倍。还观察到，添加来自胸腺或脾脏的贴壁细胞可显著加速T细胞的发育。
