转录因子TCF-1和GATA-3参与胸腺中T细胞发育最早阶段的起始过程。
Involvement of transcription factors TCF-1 and GATA-3 in the initiation of the earliest step of T cell development in the thymus.
作者信息
Hattori N, Kawamoto H, Fujimoto S, Kuno K, Katsura Y
机构信息
Department of Immunology, Kyoto University, Japan.
出版信息
J Exp Med. 1996 Sep 1;184(3):1137-47. doi: 10.1084/jem.184.3.1137.
Abstract
Flow cytometric and immunocytochemical analyses of murine fetal thymus (FT) cells with antibodies to various surface markers and transcription factors reveal that the synthesis of TCF-1 and GATA-3 protein begins simultaneously in a fraction of the most immature population of FT cells, which have the phenotype of CD4-CD8-CD44+CD25-. No TCF-1-producing cells is found in the fetal liver (FL). In CD44+CD25- FT cells, the production of TCF-1 is immediately followed by intracellular expression of CD3 epsilon. It is also found that the T cell development from FL, but not FT, progenitors in the FT organ culture system is severely inhibited by the addition of antisense oligonucleotides for either TCF-1 or GATA-3. These results strongly suggest that TCF-1 and GATA-3 play essential roles in the initiation of the earliest steps of T cell development in the thymus.
摘要
用针对各种表面标志物和转录因子的抗体对小鼠胎儿胸腺（FT）细胞进行流式细胞术和免疫细胞化学分析发现，TCF-1和GATA-3蛋白的合成在FT细胞中最不成熟群体的一部分中同时开始，这些细胞具有CD4-CD8-CD44+CD25-的表型。在胎儿肝脏（FL）中未发现产生TCF-1的细胞。在CD44+CD25- FT细胞中，TCF-1的产生紧接着是CD3ε的细胞内表达。还发现，在FT器官培养系统中，添加针对TCF-1或GATA-3的反义寡核苷酸会严重抑制来自FL而非FT祖细胞的T细胞发育。这些结果强烈表明，TCF-1和GATA-3在胸腺中T细胞发育最早步骤的起始中起重要作用。
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