School of Optoelectronic Information, Chongqing University of Technology , Chongqing 400054, China.
Anal Chem. 2014 Sep 16;86(18):9278-85. doi: 10.1021/ac502417a. Epub 2014 Sep 7.
A microstructured fiber Bragg grating (MSFBG) was created to accurately and simultaneously monitor the cell growth of photosynthetic bacteria (PSB) Rhodopseudomonas palustris CQK 01 and the temperature in a photobioreactor. The proposed sensor was made from an FBG unit that was separated into three regions, an unperturbed region, and two etched regions with smooth surfaces. The unperturbed grating region was employed to monitor the temperature. To eliminate the effects of the liquid concentration and temperature on the biomass, a polyimide-silica hybrid membrane was created and coated on an etched grating region to separate the liquids from the PSB; that is, this thinned region was developed to analyze the liquid concentration and temperature. Another etched grating region with a smaller diameter was used to determine the response to the temperature, biomass, and liquid concentration. In addition, two models were also presented to demonstrate accurate simultaneous measurement of the biomass and temperature. We discovered that the MSFBG sensor can rapidly and accurately determine the difference in the Bragg wavelength shifts caused by changes in the temperature, biomass, and liquid-phase concentration. The measured biomass is highly correlated with the real cell growth, with a correlation of 0.9438; the hydrogen production rate and temperature difference from metabolic heat production reached 1.97 mmol/L/h and 2.8 °C, respectively, in the PSB culture.
一种微结构光纤布拉格光栅（MSFBG）被创建，以准确且同时监测光合细菌（PSB）沼泽红假单胞菌 CQK01 的细胞生长和光生物反应器中的温度。所提出的传感器由一个 FBG 单元制成，该单元被分成三个区域，一个未扰区和两个具有光滑表面的蚀刻区。未扰光栅区用于监测温度。为了消除液体浓度和温度对生物质的影响，创建了聚酰亚胺-硅杂化膜并涂覆在蚀刻光栅区上，以将液体与 PSB 分离；也就是说，开发这个变薄区域来分析液体浓度和温度。另一个具有较小直径的蚀刻光栅区用于确定对温度、生物质和液体浓度的响应。此外，还提出了两种模型来演示对生物质和温度的准确同时测量。我们发现，MSFBG 传感器可以快速准确地确定由温度、生物质和液相浓度变化引起的布拉格波长移动的差异。测量的生物质与实际细胞生长高度相关，相关系数为 0.9438；在 PSB 培养物中，产氢率和代谢产热引起的温度差分别达到 1.97mmol/L/h 和 2.8°C。
