Zhong Nianbing, Zhao Mingfu, Li Yishan
Chongqing Key Laboratory of Modern Photoelectric Detection Technology and Instrument, Chongqing University of Technology, Chongqing 400054, China;
Key Laboratory of Low-grade Energy Utilization Technologies and Systems (Chongqing University), Ministry of Education, Chongqing 400030, China.
Biomed Opt Express. 2016 Jan 7;7(2):335-51. doi: 10.1364/BOE.7.000335. eCollection 2016 Feb 1.
To monitor biofilm growth on polydimethylsiloxane in a photobioreactor effectively, the biofilm cells and liquids were separated and measured using a sensor with two U-shaped, double-tapered, fiber-optic probes (Sen. and Ref. probes). The probes' Au-coated hemispherical tips enabled double-pass evanescent field absorption. The Sen. probe sensed the cells and liquids inside the biofilm. The polyimide-silica hybrid-film-coated Ref. probe separated the liquids from the biofilm cells and analyzed the liquid concentration. The biofilm structure and active biomass were also examined to confirm the effectiveness of the measurement using a simulation model. The sensor was found to effectively respond to the biofilm growth in the adsorption through exponential phases at thicknesses of 0-536 μm.
为了有效监测光生物反应器中聚二甲基硅氧烷上生物膜的生长情况，使用带有两个U形、双锥形光纤探头（传感探头和参比探头）的传感器对生物膜细胞和液体进行分离和测量。探头的镀金半球形尖端实现了双程倏逝场吸收。传感探头感应生物膜内部的细胞和液体。涂有聚酰亚胺-二氧化硅混合膜的参比探头将液体与生物膜细胞分离并分析液体浓度。还使用模拟模型检查了生物膜结构和活性生物量，以确认测量的有效性。结果发现，该传感器在生物膜厚度为0 - 536μm的吸附至指数生长阶段能有效响应生物膜的生长。
