Takahashi S, Noguchi Y, Kitagawa T
Acta Paediatr Jpn. 1989 Apr;31(2):127-35. doi: 10.1111/j.1442-200x.1989.tb01278.x.
We applied the Western-blot technique for qualifying fibrin/fibrinogen degradation products (FDP) subfragments and subunits. With this technique we examined urine or serum samples from patients with glomerulonephritis and disseminated intravascular coagulation (DIC), in order to observe how primary or secondary fibrinolysis functioned under these pathological conditions. We also tested the antigenic recognition of several FDP antibodies with this technique. The results obtained were as follows: 1) FDP in serum samples from patients with DIC consisted of both fibrinogenolytic and fibrinolytic products; 2) Fibrinolysis was predominant in the serum from one patient with Henoch-Schönlein purpura nephritis, and its level increased after fibrinolytic therapy with urokinase; 3) Fibrinogen and fibrin polymers were always the major components of urinary FDP, although fibrinolytic products such as subfragment D-D dimer were detected in patients with glomerular disease and increased in acute exacerbation; 4) Anti-FDP D-D dimer monoclonal antibody (DD 3B6), which is usually considered to react specifically with cross-linked fibrin derivatives, also seemed to have cross-reactivity with non-crosslinked fibrin derivatives.
我们应用蛋白质印迹技术来鉴定纤维蛋白/纤维蛋白原降解产物（FDP）的亚片段和亚单位。运用该技术，我们检测了肾小球肾炎和弥散性血管内凝血（DIC）患者的尿液或血清样本，以观察在这些病理状况下原发性或继发性纤溶功能是如何发挥作用的。我们还用该技术检测了几种FDP抗体的抗原识别情况。获得的结果如下：1）DIC患者血清样本中的FDP由纤维蛋白原降解产物和纤维蛋白降解产物组成；2）一名过敏性紫癜肾炎患者的血清中纤溶作用占主导，在用尿激酶进行纤溶治疗后其水平升高；3）纤维蛋白原和纤维蛋白聚合物始终是尿FDP的主要成分，尽管在肾小球疾病患者中检测到了诸如D - D二聚体亚片段等纤维蛋白降解产物，且在急性加重期有所增加；4）通常认为能与交联纤维蛋白衍生物特异性反应的抗FDP D - D二聚体单克隆抗体（DD 3B6），似乎也与非交联纤维蛋白衍生物有交叉反应。
