Chiu F C, Barnes E A, Das K, Haley J, Socolow P, Macaluso F P, Fant J
Saul R. Korey Department of Neurology, Albert Einstein College of Medicine, Bronx, New York 10461.
Neuron. 1989 May;2(5):1435-45. doi: 10.1016/0896-6273(89)90189-x.
A 66 kd protein, pl 5.4, was purified from the Triton-insoluble fraction of rat spinal cord. This protein formed 10 nm filaments in vitro. The 66 kd protein was unique, although it shared homology with the 70 kd neurofilament protein (NF-L) and vimentin. An antiserum (anti-66) specific to the 66 kd protein did not cross-react with any of the neurofilament triplet proteins. In the spinal cord, anti-66 intensely stained the axons of the anterior and lateral columns. However, afferents from dorsal root ganglia and the efferents from the motoneurons were negative. In the cerebellum, anti-66 intensely stained most axons. The 66 kd protein was readily detectable in homogenates of forebrain, cerebellum, brainstem, and spinal cord, but was found only in trace amounts in adult sciatic nerves and was not found in extraneural tissues. The 66 kd protein constituted 0.5% of total protein in the spinal cord, whereas NF-L constituted about 1.5%.
一种分子量为66kd、等电点为5.4的蛋白质从大鼠脊髓的 Triton 不溶性组分中纯化得到。该蛋白质在体外形成10nm 的细丝。66kd 蛋白质是独特的，尽管它与70kd 神经丝蛋白（NF-L）和波形蛋白具有同源性。针对66kd 蛋白质的特异性抗血清（抗-66）不与任何神经丝三联体蛋白发生交叉反应。在脊髓中，抗-66 强烈染色前柱和侧柱的轴突。然而，背根神经节的传入纤维和运动神经元的传出纤维呈阴性。在小脑中，抗-66 强烈染色大多数轴突。66kd 蛋白质在前脑、小脑、脑干和脊髓的匀浆中很容易检测到，但在成年坐骨神经中仅微量存在，在神经外组织中未发现。66kd 蛋白质占脊髓总蛋白的0.5%，而 NF-L 约占1.5%。
