Zheng Ji, Tang Jinyuan, Yin Supei, Shen Xuecheng, Zhou Zhansong
Department of Urology, Urological Surgery Research Institute, Southwest Hospital, Third Military Medical University, Chongqing, China.
Department of Urology, Urological Surgery Research Institute, Southwest Hospital, Third Military Medical University, Chongqing, China.
Urology. 2014 Sep;84(3):731.e9-13. doi: 10.1016/j.urology.2014.05.038.
To compare the results of polymerase chain reaction (PCR) and immunologic methods for the detection of nanobacteria (NB) in the expressed prostatic secretions (EPSs) of patients with type-III prostatitis.
In total, 150 patients with type-III prostatitis for whom conventional clinical treatment had failed were selected from September 2009 to April 2010. The EPS of each patient was divided into 3 parts, which were used for PCR analysis, indirect immunofluorescence staining (IIFS), and culture and subsequent indirect immunofluorescence staining (CIIFS).
PCR analysis has a higher sensitivity than IIFS for the detection of NB in EPSs. Of 83 CIIFS-positive EPS samples, 79 (95.2%) were positive by PCR. Of 67 EPS samples that were negative by CIIFS, 60 (89.6%) were negative by PCR. The sensitivity of PCR for the detection of NB compared with the CIIFS method was 95.2%, with a specificity of 89.6%. The positive predictive value was 91.9%, and the negative predictive value was 93.8%. A comparative evaluation showed no statistically significant difference between PCR and CIIFS in the detection of NB in EPSs. A strong agreement in the positive and the negative results obtained by PCR and CIIFS for NB detection was found for all EPS samples.
PCR analysis has a higher sensitivity than IIFS for NB detection in type-III prostatitis. PCR can detect nanobacterial infection in type-III prostatitis equally well as CIIFS and offers significant advantages for the rapid, simple, and economical detection of nanobacterial infection in type-III prostatitis.
比较聚合酶链反应（PCR）和免疫方法检测Ⅲ型前列腺炎患者前列腺液（EPS）中纳米细菌（NB）的结果。
选取2009年9月至2010年4月间150例常规临床治疗失败的Ⅲ型前列腺炎患者。将每位患者的EPS分成3份，分别用于PCR分析、间接免疫荧光染色（IIFS）、培养及后续间接免疫荧光染色（CIIFS）。
在EPS中检测NB时，PCR分析的灵敏度高于IIFS。在83份CIIFS阳性的EPS样本中，79份（95.2%）经PCR检测为阳性。在67份CIIFS阴性的EPS样本中，60份（89.6%）经PCR检测为阴性。与CIIFS方法相比，PCR检测NB的灵敏度为95.2%，特异性为89.6%。阳性预测值为91.9%，阴性预测值为93.8%。比较评估显示，PCR和CIIFS在EPS中检测NB时无统计学显著差异。对于所有EPS样本，PCR和CIIFS检测NB的阳性和阴性结果一致性良好。
在Ⅲ型前列腺炎中，PCR分析检测NB的灵敏度高于IIFS。PCR检测Ⅲ型前列腺炎中的纳米细菌感染与CIIFS效果相当，且在快速、简便、经济地检测Ⅲ型前列腺炎中的纳米细菌感染方面具有显著优势。
