[血液中N-甲基氨基甲酰化血红蛋白作为N，N-二甲基甲酰胺暴露生物标志物的研究]

[Study of N-methylcarbamoylated hemoglobin in blood of being an exposure biomarker of N, N-dimethylformamide].

作者信息

Chen Weiguo, Ruan Zheng, Xu Chengmin, Qian Yaling

机构信息

Zhe Jiang Academy of Medical Science, Hangzhou 310013, China.

Zhe Jiang Academy of Medical Science, Hangzhou 310013, China. Email:

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2014 May;32(5):361-4.

PMID:25169092

Abstract

OBJECTIVE

To assess the value of blood N-methylcarbamoylated haemoglobin (NMHb) as a biomarker of N, N-dimethylformamide (DMF) exposure.

METHODS

Seventy-two DMF processing workers in a synthetic leather factory were included in the DMF exposure group, and 12 workers in a food factory without exposure to DMF were included in the control group. Long-time individual sampling in workplace was performed among 45 workers in the exposure group, accompanied by a questionnaire survey. Blood and urine were collected for the determination of urinary N-methylformamide (NMF), urinary creatinine, and blood NMHb. Air DMF and urinary NMF were determined by gas chromatography. NMHb in blood was degraded to 3-methyl-5-isopropylhydantoin by Edman degradation before it could be determined by gas chromatography/mass spectrometry.

RESULTS

Air DMF in workplace and NMF in post-shift urine were both correlated with NMHb in blood, and the respective regression equations were LgNMHb (nmol/g globin) = 0.32×LgDMF (mg/m(3))+1.8 (r = 0.60, P < 0.005), and LgNMHb (nmol/g globin) = 0.47×LgNMF (mg/g Cr) + 1.4 (r = 0.56, P < 0.005).

CONCLUSION

NMHb can be used as a biomarker of long-term exposure to DMF.

摘要

目的

评估血液中N - 甲基氨基甲酰化血红蛋白（NMHb）作为N，N - 二甲基甲酰胺（DMF）暴露生物标志物的价值。

方法

将某合成皮革厂的72名DMF加工工人纳入DMF暴露组，将某未接触DMF的食品厂的12名工人纳入对照组。对暴露组中的45名工人进行工作场所长期个体采样，并同时进行问卷调查。采集血液和尿液以测定尿N - 甲基甲酰胺（NMF）、尿肌酐和血液NMHb。采用气相色谱法测定空气中的DMF和尿中的NMF。血液中的NMHb经埃德曼降解法降解为3 - 甲基 - 5 - 异丙基乙内酰脲后，再用气相色谱/质谱法进行测定。

结果

工作场所空气中的DMF和下班后尿液中的NMF均与血液中的NMHb相关，各自的回归方程分别为LgNMHb（nmol/g珠蛋白） = 0.32×LgDMF（mg/m³） + 1.8（r = 0.60，P < 0.005），以及LgNMHb（nmol/g珠蛋白） = 0.47×LgNMF（mg/g Cr） + 1.4（r = 0.56，P < 0.005）。