Zhang Ning, Fang Peng, Gu Zong-Jiang
1 Department of Immunology, School of Medicine, Soochow University , Suzhou, China .
Monoclon Antib Immunodiagn Immunother. 2014 Aug;33(4):270-4. doi: 10.1089/mab.2013.0082.
B7-H4, a member of B7 family, is widely expressed in tumor tissues and plays an important role in the negative regulation of T cell immunity. In this study, we report on the establishment and characterization of a functional anti-human B7-H4 monoclonal antibody (MAb) 5G3 through hybridoma method. Flow cytometry analysis showed that MAb 5G3 specifically bound to B7-H4 molecule. Functional experiments indicated that MAb 5G3 could block the inhibitory role of B7-H4 molecule on A549 cells in and reduce the apoptosis of Jurkat cells, suggesting that MAb 5G3 is an antagonistic antibody and a useful tool for further studies of B7-H4 functions in cancers.
B7-H4是B7家族的一员，在肿瘤组织中广泛表达，在T细胞免疫的负调控中发挥重要作用。在本研究中，我们报告了通过杂交瘤方法建立并鉴定一种功能性抗人B7-H4单克隆抗体（MAb）5G3的过程。流式细胞术分析表明，MAb 5G3特异性结合B7-H4分子。功能实验表明，MAb 5G3可阻断B7-H4分子对A549细胞的抑制作用，并减少Jurkat细胞的凋亡，这表明MAb 5G3是一种拮抗性抗体，是进一步研究B7-H4在癌症中功能的有用工具。
