State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Clin Exp Med. 2011 Sep;11(3):163-70. doi: 10.1007/s10238-010-0125-2. Epub 2010 Dec 29.
B7-H4, a new member of the B7 family, may participate in the negative regulation of cell-mediated immunity, while aberrant B7-H4 expression is detected in some tumors and it participates in the occurrence and development of the tumors. In this study, we developed one monoclonal antibody (mAb) whose clone No. was 4H8 against the extracellular domains of B7-H4 through immunization of Balb/c mice with the 3T3-mB7-H4 cells that expressed extrinsic B7-H4 stably. And we detected the expression characteristics of B7-H4 in various tumors using 4H8 mAb by enzyme-linked immunosorbent assay (ELISA), immunoprecipitation and immunohistochemistry (IHC) analysis. B7-H4 expression was significantly higher in the tumor tissues derived from uterus, breast, and colon than in their corresponding normal tissues. Further, the B7-H4 expression was related to the stage of the tumors. In contrast, B7-H4 expression did not differ significantly between the tumor tissues derived from the stomach and liver and the normal tissues. Different expression levels of B7-H4 in the tumors indicated that B7-H4 may be involved in tumor formation and development. Specific mAbs against B7-H4 will be useful in studying the role of B7-H4 in tumor pathogenesis and pathological process.
B7-H4 是 B7 家族的新成员，可能参与细胞介导的免疫负调节，而在一些肿瘤中检测到异常的 B7-H4 表达，它参与肿瘤的发生和发展。在这项研究中，我们通过用稳定表达外源性 B7-H4 的 3T3-mB7-H4 细胞免疫 Balb/c 小鼠，开发了一种针对 B7-H4 细胞外结构域的单克隆抗体（mAb），其克隆号为 4H8。我们通过酶联免疫吸附试验（ELISA）、免疫沉淀和免疫组织化学（IHC）分析，用 4H8 mAb 检测了各种肿瘤中 B7-H4 的表达特征。B7-H4 在源自子宫、乳房和结肠的肿瘤组织中的表达明显高于相应的正常组织。此外，B7-H4 的表达与肿瘤的分期有关。相比之下，源自胃和肝的肿瘤组织与正常组织之间的 B7-H4 表达没有显著差异。肿瘤中 B7-H4 的不同表达水平表明 B7-H4 可能参与肿瘤的形成和发展。针对 B7-H4 的特异性 mAb 将有助于研究 B7-H4 在肿瘤发病机制和病理过程中的作用。
