Lel Rency, Ngaira Jane, Lihana Raphael, Khamadi Samoel
1 Centre for Virus Research, Kenya Medical Research Institute , Nairobi, Kenya .
AIDS Res Hum Retroviruses. 2014 Dec;30(12):1236-8. doi: 10.1089/AID.2014.0158.
To determine HIV-1 subtypes and transmitted HIV-1 drug-resistant mutations among HIV-1-positive children born to HIV-positive mothers in Busia County, blood samples were collected from 53 children aged between 6 weeks and 5 years in 2011. Their mothers were HIV-1 positive and on antiretroviral therapy at the time the children were born. The samples were analyzed for HIV-1 drug resistance and subtypes through sequencing of portions of the HIV-1 pol gene. The generated sequences were analyzed for subtype diversity using the REGA and BLAST subtyping tools. HIV-1 drug resistance was determined using the Stanford University HIV database. Of the 53 samples that were successfully amplified and sequenced, 69.8% (37/53) were determined to be HIV-1 subtype A, 22.6% (12/53) were subtype D, 5.6% (3/53) were subtype C, and 1.8% (1/53) were subtype A1C. The prevalence of HIV-1 drug resistance mutations of any kind was 22.6% (12/53).
为了确定布西亚县感染HIV-1的母亲所生的HIV-1阳性儿童中的HIV-1亚型以及传播的HIV-1耐药突变，2011年从53名年龄在6周至5岁之间的儿童中采集了血样。这些儿童的母亲在孩子出生时HIV-1呈阳性且正在接受抗逆转录病毒治疗。通过对HIV-1 pol基因部分进行测序，对样本进行HIV-1耐药性和亚型分析。使用REGA和BLAST分型工具对生成的序列进行亚型多样性分析。利用斯坦福大学HIV数据库确定HIV-1耐药性。在成功扩增并测序的53个样本中，69.8%（37/53）被确定为HIV-1 A亚型，22.6%（12/53）为D亚型，5.6%（3/53）为C亚型，1.8%（1/53）为A1C亚型。任何类型的HIV-1耐药突变的流行率为22.6%（12/53）。
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