Tateishi Ken, Kasahara Yuichi, Watanabe Kazuyo, Hosokawa Nobuo, Doi Hiroyasu, Nakajima Kaori, Adachi Hayamitsu, Nomoto Akio
National Institute of Agrobiological Sciences, 2-1-2 Kan-non dai, Tsukuba, Ibaraki, 305-8602, Japan,
In Vitro Cell Dev Biol Anim. 2015 Jan;51(1):15-8. doi: 10.1007/s11626-014-9808-4. Epub 2014 Aug 30.
A new cell line, designated NIAS-SL64, was established from the fat body of the fifth instar larvae of the common cutworm Spodoptera litura. NIAS-SL64 cells grew as spindle-shaped and non-adherent cells in the insect-specific cell culture medium MGM-450 supplemented with 10% fetal bovine serum. Criterions for the establishment of the NIAS-SL64 cell line is spindle shape and length (30~90 μm) stabilized after 100 passages. The doubling time of the cells was 24 h at 25°C. Lipopolysaccharide significantly stimulated the release of lysozyme activity by NIAS-SL64 cells. Lysozyme is one of the components of the innate immunity and plays important role as lytic enzyme in infection. Lysozyme activity released from NIAS-SL64 would be a marker for immune response. The released lysozyme activity critically depends on morphology of the cells and would be a criterion of the establishment of the cell line. Lysozyme activity was suppressed in a dose-dependent manner by the immunosuppressive agent cyclosporin A.
一种名为NIAS-SL64的新细胞系，是从斜纹夜蛾五龄幼虫的脂肪体中建立的。NIAS-SL64细胞在添加了10%胎牛血清的昆虫特异性细胞培养基MGM-450中呈纺锤形且不贴壁生长。NIAS-SL64细胞系建立的标准是传代100次后纺锤形和长度（30~90μm）稳定。细胞在25°C时的倍增时间为24小时。脂多糖显著刺激NIAS-SL64细胞释放溶菌酶活性。溶菌酶是固有免疫的组成部分之一，在感染中作为裂解酶发挥重要作用。NIAS-SL64释放的溶菌酶活性将是免疫反应的一个标志物。释放的溶菌酶活性严重依赖于细胞形态，将是细胞系建立的一个标准。免疫抑制剂环孢素A以剂量依赖的方式抑制溶菌酶活性。
