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A new cell line from the wax moth Galleria mellonella Linne (Lepidoptera: Pyralididae).

作者信息

Eguchi Daisuke, Iwabuchi Kikuo

机构信息

Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509 Japan.

出版信息

In Vitro Cell Dev Biol Anim. 2006 Jan-Feb;42(1-2):1-3. doi: 10.1007/s11626-006-0002-1.

DOI:10.1007/s11626-006-0002-1
PMID:16618204
Abstract

A cell line derived from the larval-fat body tissues of the wax moth, Galleria mellonella Linne, was established in MGM-450 medium. The cells grew in suspension and were mainly spherical in shape. Population doubling time was between 1.4 and 1.7 d over a range of 15 to 35 degrees C, and the maximum growth rate was at 25 degrees C. The chromosome number ranged from 70-239, with a mode of 170. The cells were sensitive to 20-hydroxyecdysone, which stimulated their growth and induced morphological changes. The cell line was designated GaMe-LF1.

摘要

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本文引用的文献

1
Studies on insect tissue culture. I. Culture of tissues from the wax moth, Galleria mellonella, L. in vitro.昆虫组织培养研究。I. 大蜡螟(Galleria mellonella, L.)组织的体外培养。
Folia Biol (Praha). 1961 Dec;7:400-8.
2
Growth of an ovarian cell line of Galleria mellonella and its response to immune-inducing factors.大蜡螟卵巢细胞系的生长及其对免疫诱导因子的反应。
In Vitro Cell Dev Biol Anim. 2002 Nov-Dec;38(10):572-81. doi: 10.1290/1543-706x(2002)38<572:goaocl>2.0.co;2.
3
An established cell line from the beetle, Xylotrechus pyrrhoderus (Coleoptera: Cerambycidae).
一种源自甲虫(梨眼天牛,鞘翅目:天牛科)的成熟细胞系。
In Vitro Cell Dev Biol Anim. 1999 Nov-Dec;35(10):612-5. doi: 10.1007/s11626-999-0100-y.
4
A new small RNA virus persistently infecting an established cell line of Galleria mellonella, induced by a heterologous infection.一种由异源感染诱导的、持续感染已建立的大蜡螟细胞系的新型小RNA病毒。
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