Djelić Ninoslav, Radaković Milena, Spremo-Potparević Biljana, Zivković Lada, Bajić Vladan, Stevanović Jevrosima, Stanimirović Zoran
Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Oslobodjenja Blvd. 18, Belgrade, Serbia.
Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Oslobodjenja Blvd. 18, Belgrade, Serbia.
Toxicol In Vitro. 2015 Feb;29(1):27-33. doi: 10.1016/j.tiv.2014.08.001. Epub 2014 Aug 27.
Catechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to oxidative damage of cellular macromolecules including DNA. The objective of this investigation was to evaluate possible genotoxic effects of a natural catecholamine adrenaline in cultured human lymphocytes using cytogenetic (sister chromatid exchange and micronuclei) and the single cell gel electrophoresis (Comet) assay. In cytogenetic tests, six experimental concentrations of adrenaline were used in a range from 0.01-500 μM. There were no indications of genotoxic effects of adrenaline in sister chromatid exchange and micronucleus tests. However, at four highest concentrations of adrenaline (5 μM, 50 μM, 150 μM and 300 μM) we observed a decreased mitotic index and cell-cycle delay. In addition, in the Comet assay we used adrenaline in a range from 0.0005-500 μM, at two treatment times: 15 min or 60 min. In contrast to cytogenetic analysis, there was a dose-dependent increase of DNA damage detected in the Comet assay. These effects were significantly reduced by concomitant treatment with quercetin or catalase. Therefore, the obtained results indicate that adrenaline may exhibit genotoxic effects in cultured human lymphocytes, most likely due to production of reactive oxygen species.
儿茶酚基团可参与氧化还原循环，同时产生活性氧（ROS），这可能导致包括DNA在内的细胞大分子发生氧化损伤。本研究的目的是使用细胞遗传学方法（姐妹染色单体交换和微核试验）以及单细胞凝胶电泳（彗星试验），评估天然儿茶酚胺肾上腺素对培养的人淋巴细胞可能产生的遗传毒性作用。在细胞遗传学试验中，使用了六种浓度范围为0.01 - 500μM的肾上腺素进行实验。在姐妹染色单体交换和微核试验中，没有迹象表明肾上腺素具有遗传毒性作用。然而，在肾上腺素的四个最高浓度（5μM、50μM、150μM和300μM）下，我们观察到有丝分裂指数降低和细胞周期延迟。此外，在彗星试验中，我们使用了浓度范围为0.0005 - 500μM的肾上腺素，处理时间为15分钟或60分钟。与细胞遗传学分析不同，在彗星试验中检测到DNA损伤呈剂量依赖性增加。用槲皮素或过氧化氢酶同时处理可显著降低这些效应。因此，所得结果表明，肾上腺素可能对培养的人淋巴细胞表现出遗传毒性作用，最有可能是由于产生活性氧所致。
