Faculty of Science, Department of Biology, Genetic Toxicology Laboratory, Gazi University, 06500 Teknikokullar, Ankara, Turkey.
Food Chem Toxicol. 2013 Jun;56:240-6. doi: 10.1016/j.fct.2013.02.034. Epub 2013 Feb 26.
The genotoxic potential of the vaccine adjuvant Squalene was assessed by the chromosomal aberrations (CAs), sister chromatid exchanges (SCEs) and micronucleus (MNs) tests in human lymphocytes and comet assay in both human and rat lymphocytes. Five different concentrations of squalene (1250-20,000 μg/ml for human lymphocytes and 0.07-1.12 mg/kg for rat lymphocytes) were studied. Squalene did not affect the CAs and MN frequency, in all treatments in vitro. A significant increase in SCEs was observed in almost all concentrations at 24 h treatment. Squalene did not affect significantly the comet tail length (CTL) (except 2500 μg/ml) and comet tail intensity (CTI) at all treatments in vitro. In rats, squalene significantly increased and decreased CTL and CTI in some doses. Although there are increasing and reduction in the effect, squalene cannot be regarded as genotoxic in human lymphocytes. However, further in vivo studies are required to be sure on the effect.
该疫苗佐剂角鲨烯的遗传毒性通过染色体畸变（CA）、姐妹染色单体交换（SCE）和微核（MN）试验在人淋巴细胞和彗星试验在人和大鼠淋巴细胞中进行评估。研究了 5 种不同浓度的角鲨烯（1250-20000μg/ml 用于人淋巴细胞和 0.07-1.12mg/kg 用于大鼠淋巴细胞）。角鲨烯在体外所有处理中均未影响 CA 和 MN 频率。在 24 小时处理时，几乎所有浓度均观察到 SCE 显著增加。角鲨烯在体外所有处理中均未显著影响彗星尾长（CTL）（2500μg/ml 除外）和彗星尾强度（CTI）。在大鼠中，角鲨烯在某些剂量下显著增加和降低 CTL 和 CTI。尽管效果有增加和减少，但角鲨烯不能被认为在人淋巴细胞中具有遗传毒性。然而，需要进一步的体内研究来确定其效果。
