单酚单加氧酶形成黑色素的立体化学研究。

Stereochemical studies on the formation of melanin by monophenol monooxygenase.

作者信息

Kajiwara M, Kurumaya K, Kohno Y, Tomita K, Carpenter A T

出版信息

Chem Pharm Bull (Tokyo). 1989 Dec;37(12):3386-9. doi: 10.1248/cpb.37.3386.

DOI:10.1248/cpb.37.3386

Abstract

The oxidation of tyrosine by monophenol monooxygenase (tyrosinase: EC 1.10.3.1) to melanin has been studied by a combination of ultraviolet, circular dichroism, and nuclear magnetic resonance techniques. It is demonstrated that the chiral intermediate (dopachrome) is generated stereoselectively in this enzymic reaction.

摘要

通过紫外、圆二色性和核磁共振技术相结合的方法，对单酚单加氧酶（酪氨酸酶：EC 1.10.3.1）将酪氨酸氧化为黑色素的过程进行了研究。结果表明，在该酶促反应中立体选择性地生成了手性中间体（多巴色素）。