Hayasaka S, Tsuchiya M, Noda S, Setogawa T, Mizuno K
Department of Ophthalmology, Shimane Medical University, Japan.
Ophthalmic Res. 1988;20(6):376-9. doi: 10.1159/000266755.
We examined spectrophotometrically the ability of drugs to bind with dopa-auto-oxidation melanin or tyrosinase-catalyzed dopa-melanin. Each drug, at 5 X 10(-5) M, was incubated at 37 degrees C for 8 h with 0.4 mg of synthetic melanins in 20 mM potassium phosphate buffer at pH 7.0 or in 20 mM acetate buffer at pH 4.8. Chloroquine, thioridazine, befunolol, pindolol, daunomycin and 5-fluorouracil bound to melanin at pH 7.0 and 4.8. Methotrexate bound to melanin at pH 4.8, but not at pH 7.0. Pilocarpine, epinephrine, acyclovir, vincristine and colchicine did not bind to dopa-auto-oxidation melanin or tyrosinase-catalyzed dopa-melanin.
我们用分光光度法检测了药物与多巴自氧化黑色素或酪氨酸酶催化的多巴黑色素结合的能力。每种药物在5×10⁻⁵M浓度下，于37℃在pH 7.0的20 mM磷酸钾缓冲液或pH 4.8的20 mM乙酸盐缓冲液中，与0.4 mg合成黑色素孵育8小时。氯喹、硫利达嗪、倍他洛尔、吲哚洛尔、柔红霉素和5-氟尿嘧啶在pH 7.0和4.8时与黑色素结合。甲氨蝶呤在pH 4.8时与黑色素结合，但在pH 7.0时不结合。毛果芸香碱、肾上腺素、阿昔洛韦、长春新碱和秋水仙碱不与多巴自氧化黑色素或酪氨酸酶催化的多巴黑色素结合。
