miR-339-5p 通过调控 NF-κB 通路抑制酒精诱导的脑炎症。
miR-339-5p inhibits alcohol-induced brain inflammation through regulating NF-κB pathway.
机构信息
Department of Neurology, The First Affiliated School of Harbin Medical University, Harbin 150001, China.
Department of Neurology, Heilongjiang Provincial Hospital, Harbin 150036, China.
出版信息
Biochem Biophys Res Commun. 2014 Sep 26;452(3):450-6. doi: 10.1016/j.bbrc.2014.08.092. Epub 2014 Aug 27.
Alcohol-induced neuroinflammation is mediated by the innate immunesystem. Pro-inflammatory responses to alcohol are modulated by miRNAs. The miRNA miR-339-5p has previously been found to be upregulated in alcohol-induced neuroinflammation. However, little has been elucidated on the regulatory functions of this miRNA in alcohol-induced neuroinflammation. We investigated the function of miR-339-5p in alcohol exposed brain tissue and isolated microglial cells using ex vivo and in vitro techniques. Our results show that alcohol induces transcription of miR 339-5p, IL-6, IL-1β and TNF-α in mouse brain tissue and isolated microglial cells by activating NF-κB. Alcohol activation of NF-κB allows for nuclear translocation of the NF-κB subunit p65 and expression of pro-inflammatory mediators. miR-339-5p inhibited expression of these pro-inflammatory factors through the NF-κB pathway by abolishing IKK-β and IKK-ε activity.
酒精引起的神经炎症是由先天免疫系统介导的。miRNA 调节对酒精的促炎反应。miR-339-5p 先前被发现在上调酒精诱导的神经炎症中。然而，miR-339-5p 在酒精诱导的神经炎症中的调节功能还不太清楚。我们使用离体和体外技术研究了 miR-339-5p 在酒精暴露的脑组织和分离的小胶质细胞中的功能。我们的结果表明，酒精通过激活 NF-κB 诱导小鼠脑组织和分离的小胶质细胞中 miR 339-5p、IL-6、IL-1β 和 TNF-α 的转录。NF-κB 的激活允许 NF-κB 亚基 p65 和促炎介质的核易位。miR-339-5p 通过抑制 IKK-β 和 IKK-ε 的活性，通过 NF-κB 途径抑制这些促炎因子的表达。
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