埃及伊蚊唾液蛋白“埃及伊蚊素”共同接种可调节脊椎动物宿主中的登革病毒感染。
Aedes aegypti salivary protein "aegyptin" co-inoculation modulates dengue virus infection in the vertebrate host.
作者信息
McCracken M K, Christofferson R C, Grasperge B J, Calvo E, Chisenhall D M, Mores C N
机构信息
Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Skip Bertman Drive, Baton Rouge, LA 70803, United States.
Laboratory of Malaria and Vector Research, National Institutes of Health, 12735 Twinbrook Parkway, Rockville, MD 20852, United States.
出版信息
Virology. 2014 Nov;468-470:133-139. doi: 10.1016/j.virol.2014.07.019. Epub 2014 Aug 28.
Dengue virus (DENV) is transmitted in the saliva of the mosquito vector Aedes aegypti during blood meal acquisition. This saliva is composed of numerous proteins with the capacity to disrupt hemostasis or modulate the vertebrate immune response. One such protein, termed "aegyptin," is an allergen and inhibitor of clot formation, and has been found in decreased abundance in the saliva of DENV-infected mosquitoes. To examine the influence of aegyptin on DENV infection of the vertebrate, we inoculated IRF-3/7(-/- -/-) mice with DENV serotype 2 strain 1232 with and without co-inoculation of aegyptin. Mice that received aegyptin exhibited decreased DENV titers in inoculation sites and in circulation, as well as increased concentrations of GM-CSF, IFN-γ, IL-5, and IL-6, at 48 h post-inoculation when compared to mice that received inoculation of DENV alone. These and other data suggest that aegyptin impacts DENV perpetuation via elevated induction of the immune response.
登革病毒（DENV）在埃及伊蚊媒介获取血液时通过其唾液传播。这种唾液由多种蛋白质组成，这些蛋白质能够破坏止血或调节脊椎动物的免疫反应。一种名为“埃及伊蚊素”的蛋白质就是其中之一，它是一种过敏原和凝血形成抑制剂，并且在感染登革病毒的蚊子唾液中含量降低。为了研究埃及伊蚊素对脊椎动物登革病毒感染的影响，我们用登革病毒2型毒株1232对IRF-3/7（-/- -/-）小鼠进行接种，接种时分别添加和不添加埃及伊蚊素。与仅接种登革病毒的小鼠相比，接种了埃及伊蚊素的小鼠在接种后48小时，接种部位和循环中的登革病毒滴度降低，同时GM-CSF、IFN-γ、IL-5和IL-6的浓度升高。这些数据以及其他数据表明，埃及伊蚊素通过增强免疫反应的诱导来影响登革病毒的持续存在。
Zotero 插件