Londoño-Rentería Berlín, Cárdenas Jenny C, Giovanni Jennifer E, Cárdenas Lucio, Villamizar Paloma, Rolón Jennifer, Chisenhall Daniel M, Christofferson Rebecca C, Carvajal Daisy J, Pérez Omar G, Wesson Dawn M, Mores Christopher N
Grupo de Investigaciones en Enfermedades Parasitarias e Infecciosas, Universidad de Pamplona, Norte de Santander, Colombia.
Hospital Local Los Patios, Norte de Santander, Colombia.
Biomedica. 2015 Oct-Dec;35(4):572-81. doi: 10.7705/biomedica.v35i4.2530.
Mosquito salivary proteins are able to induce an antibody response that reflects the level of human-vector contact. IgG antibodies against dengue virus (DENV-IgG) are indicators of previous exposure. The risk of DENV transmission is not only associated to mosquito or dengue factors, but also to socioeconomic factors that may play an important role in the disease epidemiology.
To determine the effect of the presence of Aedes aegypti mosquitos in different stages in households and the history of dengue exposure on vector-human contact determined by the level of anti-salivary protein antibodies in people living in a Colombian endemic area.
A pilot study of 58 households and 55 human subjects was conducted in Norte de Santander, Colombia. A questionnaire for socioeconomic factors was administered and houses were examined for the presence of Ae. aegypti specimens in the aquatic stages. The level of DENV-IgG antibodies (DENV-IgG), in addition to IgG and IgM anti- Ae. aegypti salivary gland extract (SGE) antibodies (SGE-IgG, SGE-IgM) were evaluated by ELISA using blood collected in filter paper.
We found a significant higher level of SGE-IgG antibodies in subjects living in houses with Ae. aegypti in aquatic stages. We also found a higher concentration of SGE-IgG antibodies in people exposed to DENV, a positive correlation between IgM-SGE and IgG-DENV and a negative correlation with IgG-SGE.
Anti-salivary proteins antibodies are consistent with the presence of Ae. aegypti aquatic stages inside houses and DENV-IgG antibodies concentrations.
蚊子唾液蛋白能够引发反映人与媒介接触水平的抗体反应。抗登革病毒IgG抗体（DENV-IgG）是既往暴露的指标。登革病毒传播风险不仅与蚊子或登革热因素有关，还与可能在疾病流行病学中起重要作用的社会经济因素有关。
确定哥伦比亚流行地区家庭中不同阶段埃及伊蚊的存在情况以及登革热暴露史对通过抗唾液蛋白抗体水平测定的媒介与人类接触的影响。
在哥伦比亚北桑坦德省对58户家庭和55名受试者进行了一项试点研究。发放了关于社会经济因素的问卷，并检查房屋中是否存在处于水生阶段的埃及伊蚊标本。除了IgG和IgM抗埃及伊蚊唾液腺提取物（SGE）抗体（SGE-IgG、SGE-IgM）外，还使用滤纸采集的血液通过ELISA评估DENV-IgG抗体（DENV-IgG）水平。
我们发现，居住在有处于水生阶段埃及伊蚊房屋中的受试者，其SGE-IgG抗体水平显著更高。我们还发现，暴露于登革病毒的人群中SGE-IgG抗体浓度更高，IgM-SGE与IgG-DENV之间呈正相关，与IgG-SGE呈负相关。
抗唾液蛋白抗体与房屋内埃及伊蚊水生阶段的存在以及DENV-IgG抗体浓度一致。
