School of Veterinary Medicine, Department of Pathobiological Sciences, Louisiana State University, Baton Rouge, Louisiana; Faculty of Health, Department of Microbiology, University of Pamplona, Pamplona, Norte de Santander, Colombia.
Am J Trop Med Hyg. 2014 Mar;90(3):431-7. doi: 10.4269/ajtmh.13-0412. Epub 2014 Jan 20.
Dengue virus (DENV) is the most important mosquito-transmitted flavivirus that is transmitted throughout the tropical and subtropical regions of the world. The primary mosquito vector of DENV in urban locations is Aedes aegypti. Key to understanding the transmission of DENV is the relationship between pathogen and vector. Accordingly, we report our preliminary characterization of the differentially expressed proteins from Ae. aegypti mosquitoes after DENV infection. We investigated the virus-vector interaction through changes in the proteome of the salivary glands of mosquitoes with disseminated DENV serotype 2 (DENV-2) infections using two-dimensional gel electrophoresis and identification by mass spectrometry. Our findings indicate that DENV-2 infection in the Ae. aegypti salivary gland alters the expression of structural, secreted, and metabolic proteins. These changes in the salivary gland proteome highlight the virally influenced environment caused by a DENV-2 infection and warrant additional investigation to determine if these differences extend to the expectorated saliva.
登革病毒（DENV）是最重要的经蚊传播的黄病毒，在世界热带和亚热带地区传播。在城市地区，DENV 的主要蚊媒是埃及伊蚊。了解 DENV 传播的关键是病原体和媒介之间的关系。因此，我们报告了我们对感染 DENV 后的埃及伊蚊唾液腺中差异表达蛋白的初步表征。我们通过二维凝胶电泳和质谱鉴定，研究了具有传播性 DENV 血清型 2（DENV-2）感染的蚊子唾液腺蛋白质组的变化，从而研究了病毒-媒介相互作用。我们的研究结果表明，DENV-2 感染埃及伊蚊唾液腺会改变结构蛋白、分泌蛋白和代谢蛋白的表达。唾液腺蛋白质组的这些变化突出了 DENV-2 感染引起的病毒影响的环境，并需要进一步研究以确定这些差异是否扩展到被吐出的唾液。
