Safonova I G, Sviridov D D, Men'shikov G B, Repin V S
Biull Eksp Biol Med. 1989 Dec;108(12):666-8.
The effect of temperature and Ca2+ ions on the interaction of high-density lipoprotein (HDL3) with human enterocytes was studied. It was shown that Kd measured at 4 degrees C is similar to that at 37 degrees C. Maximal number of binding sites at 37 degrees C is 15-fold times higher than that at 4 degrees C. EDTA (10 mM) and CaCl2 (0.5-5 mM) did not affect binding and uptake of HDL3 by human enterocytes. The obtained results indicate that HDL3-binding with these cells depends on temperature and does not depend on Ca2+ ions.
研究了温度和钙离子对高密度脂蛋白（HDL3）与人肠上皮细胞相互作用的影响。结果表明，在4℃测得的解离常数（Kd）与37℃时相似。37℃时结合位点的最大数量比4℃时高15倍。10 mM的乙二胺四乙酸（EDTA）和0.5 - 5 mM的氯化钙（CaCl2）不影响人肠上皮细胞对HDL3的结合和摄取。所得结果表明，HDL3与这些细胞的结合取决于温度，而不依赖于钙离子。
