Sulphate-stimulate liver glycogen phosphorylase b: new evidence of changes in the conformation of the enzyme.

It has been known for same time that sulphate ions stimulate liver glycogen phosphorylase b, both in the presence and in the absence of AMP. In the present paper we describe some observations (like a modified method of purification of the enzyme after sulphate treatment of the animals) suggesting that actual changes of the physical properties of the enzyme occur after intravenous injection of sodium sulphate. In order to avoid formation of phosphorylase a these studies were performed on enzyme from phosphorylase-b-kinase deficient (gsd/gsd) rats.