Soh Keng Cher, Hatzimanikatis Vassily
École Polytechnique Fédérale de Lausanne (EFPL), Lausanne, Switzerland.
Methods Mol Biol. 2014;1191:49-63. doi: 10.1007/978-1-4939-1170-7_3.
Flux balance analysis of stoichiometric metabolic models has become one of the most common methods for estimating intracellular fluxes. However most of these networks are underdetermined and can have multiple alternate optimal flux distributions. Thermodynamic constraints can reduce the solution space significantly and at the same time provide a platform for the integration of metabolomics data. Here we go through the procedure to incorporate thermodynamic constraints and perform thermodynamic analysis of metabolic networks.
化学计量代谢模型的通量平衡分析已成为估计细胞内通量最常用的方法之一。然而，这些网络大多是欠定的，可能有多个交替的最优通量分布。热力学约束可以显著减少解空间，同时为代谢组学数据的整合提供一个平台。在这里，我们将介绍纳入热力学约束并对代谢网络进行热力学分析的过程。
