Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pondicherry, India.
Biomed J. 2014 Nov-Dec;37(6):411-4. doi: 10.4103/2319-4170.132907.
Although conventional antibiotic susceptibility tests are most commonly performed for methicillin-resistant Staphylococcus aureus (MRSA), the results of these phenotypic tests are dependent on the standardization of the culture conditions. The aim of the study was to evaluate the conventional phenotypic screening tests in comparison to the mecA gene polymerase chain reaction (PCR). One hundred and two clinical isolates of MRSA identified by the oxacillin disk diffusion were subjected to PCR for the mecA gene and by the cefoxitin disk diffusion test and culture on oxacillin screen agar, mannitol salt agar, and methicillin-resistant Staphylococcus aureus Agar (MeReSA) selective medium, for MRSA. Although all 102 isolates were resistant in oxacillin and cefoxitin disk diffusion, 92 (90.1%) isolates were positive for the mecA gene. The sensitivities of the mannitol salt agar, MeReSA agar, and oxacillin screen agar were 89.13, 97.82, and 98.91%, respectively. The oxacillin screen agar may be recommended for confirming methicillin resistance in the disk diffusion test in resource-poor settings, where molecular methods are not available.
虽然传统的抗生素药敏试验最常用于耐甲氧西林金黄色葡萄球菌（MRSA），但这些表型试验的结果取决于培养条件的标准化。本研究的目的是评估常规表型筛选试验与 mecA 基因聚合酶链反应（PCR）的比较。102 株经苯唑西林纸片扩散法鉴定的耐甲氧西林金黄色葡萄球菌临床分离株进行 mecA 基因 PCR 检测，以及头孢西丁纸片扩散试验和在苯唑西林筛选琼脂、甘露醇盐琼脂和耐甲氧西林金黄色葡萄球菌琼脂（MeReSA）选择性培养基上培养，以检测耐甲氧西林金黄色葡萄球菌。尽管所有 102 株分离株在苯唑西林和头孢西丁纸片扩散中均有耐药性，但 92（90.1%）株分离株 mecA 基因阳性。甘露醇盐琼脂、MeReSA 琼脂和苯唑西林筛选琼脂的敏感性分别为 89.13%、97.82%和 98.91%。在资源匮乏的环境中，无法进行分子方法检测时，苯唑西林筛选琼脂可能有助于确认纸片扩散试验中的耐甲氧西林。
Zotero 插件