Frequency of Aminoglycoside-Resistance Genes in Methicillin-Resistant (MRSA) Isolates from Hospitalized Patients.

BACKGROUND

is one of the most important causative agents in community- and hospital-acquired infections. Aminoglycosides are powerful bactericidal drugs that are often used in combination with beta-lactams or glycopeptides to treat staphylococcal infections.

OBJECTIVES

The main objective of the present study was to determine the prevalence of aminoglycoside resistance among methicillin-resistant (MRSA) isolates in hospitalized patients in Sari and Tehran, Iran.

METHODS

In this study, 174 MRSA strains isolated from different clinical samples, such as blood, sputum, tracheal exudates, bronchus, pleura, urine, wounds, and catheters, were collected from hospitalized patients in Tehran and Sari during 2014. Antibiotic susceptibility testing was performed against nine antibiotics with the Kirby-Bauer disk diffusion method according to CLSI guidelines. The MRSA strains were examined with oxacillin and cefoxitin disks. MRSA was then validated by detection of the gene. PCR was used to evaluate the prevalence of the aminoglycoside-resistance genes , , and among the MRSA isolates.

RESULTS

The results of drug susceptibility testing showed that the highest rate of resistance was against erythromycin in Tehran (84.4%) and gentamicin (71.7%) in Sari. All isolates were sensitive to vancomycin, and all strains harbored the gene. The , , and genes were detected among 134 (77%), 119 (68.4%), and 122 (70.1%) of the isolates, respectively.

CONCLUSIONS

The present study showed a high prevalence of aminoglycoside-resistance genes among MRSA isolates in two cities in Iran.