Saenhom Natkamon, Kansan Rada, Chopjitt Peechanika, Boueroy Parichart, Hatrongjit Rujirat, Kerdsin Anusak
Faculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand.
Faculty of Science and Engineering, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand.
Heliyon. 2022 Feb 11;8(2):e08950. doi: 10.1016/j.heliyon.2022.e08950. eCollection 2022 Feb.
Methicillin-resistant staphylococci (MRS), including methicillin-resistant (MRSA) and methicillin-resistant coagulase-negative staphylococci (MRCoNS), have a global impact as a public health threat contributing significantly to morbidity, mortality, and socio-economic costs. Accurate and rapid detection of MRS results in effective antimicrobial therapy, immediate patient isolation, dissemination control, and appropriate disinfection measures. Herein, we developed an in-house cefoxitin screening broth and compared it to the cefoxitin disk diffusion method and polymerase chain reaction (PCR) for the detection of MRS. Verification of this screening broth on 52 MRSA, 37 MRCoNS, 44 methicillin-susceptible (MSSA), and 11 MSCoNS revealed greater validity for MRSA/MSSA than for MRCoNS/MSCoNS. The kappa coefficient of 0.87 was superior for determination of MRSA and MSSA, whereas it was 0.54, which was considered poor, for determination of MRCoNS and MSCoNS. Application of this assay to screen MRSA should be useful in clinical laboratories and hospital infection-control units.
耐甲氧西林葡萄球菌（MRS），包括耐甲氧西林金黄色葡萄球菌（MRSA）和耐甲氧西林凝固酶阴性葡萄球菌（MRCoNS），作为一种公共卫生威胁，在全球范围内产生影响，对发病率、死亡率和社会经济成本有重大贡献。准确快速地检测MRS可实现有效的抗菌治疗、及时的患者隔离、传播控制和适当的消毒措施。在此，我们开发了一种内部头孢西丁筛选肉汤，并将其与头孢西丁纸片扩散法和聚合酶链反应（PCR）用于检测MRS进行比较。对52株MRSA、37株MRCoNS、44株甲氧西林敏感金黄色葡萄球菌（MSSA）和11株甲氧西林敏感凝固酶阴性葡萄球菌（MSCoNS）进行该筛选肉汤验证，结果显示其对MRSA/MSSA的有效性高于对MRCoNS/MSCoNS。对于MRSA和MSSA的测定，kappa系数为0.87，表现优异；而对于MRCoNS和MSCoNS的测定，kappa系数为0.54，被认为较差。将该检测方法应用于MRSA筛查在临床实验室和医院感染控制部门应会很有用。
