McLauchlin J, Ridley A M, Taylor A G
Division of Microbiological Reagent and Quality Control, Central Public Health Laboratory, Colindale, London, Britain.
Acta Microbiol Hung. 1989;36(4):467-71.
Two monoclonal antibodies which recognise serogroup specific cell surface antigens of Listeria monocytogenes are described. The antibodies were specific for Listeria (i.e. did not react with 87 strains of bacteria from 14 other genera). In an indirect immunofluorescent test the antibodies had similar specificities when tested against strains of L. monocytogenes from human infections grown under a number of different growth conditions, and serological reference strains. A direct immunofluorescent antibody test was developed, and similar specificities of the antibodies found with strains of Listeria which had been isolated from food and grown under 2 different growth conditions. This technique is also used to directly demonstrate Listeria in soft cheese.
描述了两种识别单核细胞增生李斯特菌血清群特异性细胞表面抗原的单克隆抗体。这些抗体对李斯特菌具有特异性（即不与来自其他14个属的87株细菌发生反应）。在间接免疫荧光试验中，当针对在多种不同生长条件下培养的来自人类感染的单核细胞增生李斯特菌菌株以及血清学参考菌株进行测试时，这些抗体具有相似的特异性。开发了一种直接免疫荧光抗体试验，并且发现这些抗体对从食品中分离并在两种不同生长条件下培养的李斯特菌菌株具有相似的特异性。该技术还用于直接检测软奶酪中的李斯特菌。
