Ziegler H K, Orlin C A
Clin Invest Med. 1984;7(4):239-42.
Hybridomas secreting antibodies directed against Listeria monocytogenes were produced by the fusion of a drug sensitive tumor cell line with splenic B cells from mice immunized with L. monocytogenes. A solid phase radioimmunoassay (RIA) screening procedure was performed with three forms of listerial antigens: (i) heat killed L. monocytogenes (HKLM); (ii) isopycnic gradient purified soluble listerial proteins (SLP) and (iii) syngeneic macrophages which had ingested HKLM (MAC-LM). Ninety-three hybridoma lines were identified which showed antibody reactivity to at least one of the antigenic forms. The predominant isotypes of hybridoma derived antibodies were IgG2 and IgM. Of those antibodies reactive with HKLM 70% appeared species specific and did not react with L. grayi, L. denitrificans or L. murrayi. The others (30%) showed several cross-reactivity patterns with bacteria such as Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. Interestingly, of those positive on MAC-LM (27), 13 lines were reactive only with HKLM, 8 with all three antigenic forms, and 6 were reactive only with MAC-LM. Three lines were also identified which reacted with SLP alone. We anticipate that these antibody reagents will prove extremely useful in the identification and purification of antigenic structures and in the study of macrophage functions relating to antigen processing and presentation.
通过将一种对药物敏感的肿瘤细胞系与经单核细胞增生李斯特菌免疫的小鼠脾脏B细胞融合，产生了分泌针对单核细胞增生李斯特菌抗体的杂交瘤。采用三种形式的李斯特菌抗原进行了固相放射免疫分析（RIA）筛选程序：（i）热灭活的单核细胞增生李斯特菌（HKLM）；（ii）等密度梯度纯化的可溶性李斯特菌蛋白（SLP）；（iii）摄取了HKLM的同基因巨噬细胞（MAC-LM）。鉴定出93个杂交瘤细胞系，它们对至少一种抗原形式表现出抗体反应性。杂交瘤衍生抗体的主要亚型为IgG2和IgM。在与HKLM反应的那些抗体中，70%表现出种属特异性，不与格氏李斯特菌、反硝化李斯特菌或默氏李斯特菌反应。其他（30%）则与金黄色葡萄球菌、大肠杆菌和枯草芽孢杆菌等细菌表现出几种交叉反应模式。有趣的是，在对MAC-LM呈阳性的那些细胞系（27个）中，13个细胞系仅与HKLM反应，8个与所有三种抗原形式反应，6个仅与MAC-LM反应。还鉴定出三个仅与SLP反应的细胞系。我们预计这些抗体试剂在抗原结构的鉴定和纯化以及与抗原加工和呈递相关的巨噬细胞功能研究中将被证明极其有用。
