对来自印度东南海岸的22种软体动物门海洋无脊椎动物提取物的抗血管生成潜力进行筛选。

Screening of antiangiogenic potential of twenty two marine invertebrate extracts of phylum Mollusca from South East Coast of India.

作者信息

Gupta Pankaj, Arumugam Muthuvel, Azad Raj Vardhan, Saxena Rohit, Ghose Supriyo, Biswas Nihar Ranjan, Velpandian Thirumurthy

机构信息

Department of Ocular Pharmacology and Pharmacy, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi-110029, India.

Centre for Advanced Study in Marine Biology, Annamalai University, Parangipettai, Tamil Nadu, India.

出版信息

Asian Pac J Trop Biomed. 2014 May;4(Suppl 1):S129-38. doi: 10.12980/APJTB.4.2014C701.

DOI:10.12980/APJTB.4.2014C701

PMID:25183067

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4025331/

Abstract

OBJECTIVE

To evaluate the antiangiogenic potential of twenty two marine invertebrate species of Phylum Mollusca from south east coast of India.

METHODS

Live specimens of molluscan species were collected and their methanolic extracts were evaluated for preliminary antiangiogenic activity using the in ovo chick chorio-allantoic membrane assay. The extracts were further evaluated for in vivo antiangiogenic activity using chemical cautery induced corneal neovascularization assay in rats and oxygen induced retinopathy assay in rat pups.

RESULTS

In the chick chorio-allantoic membrane assay, four methanolic extracts of marine molluscan species viz. Meretrix meretrix, Meretrix casta, Telescopium telescopium and Bursa crumena methanolic extracts exhibited noticeable antiangiogenic activity at the tested concentration of 200 µg whereby they significantly inhibited the VEGF induced proliferation of new blood vessels. Among these four extracts, the methanolic extract of Meretrix casta exhibited relatively higher degree of antiangiogenic activity with an inhibitiory percentage (64.63%) of the VEGF induced neovascularization followed by the methanolic extracts of Telescopium telescopium (62.02%), Bursa crumena (60.48%) and Meretrix meretrix (47.01%). These four methanolic extracts were further evaluated for in vivo antiangiogenic activity whereby the methanolic extract of Telescopium telescopium exhibited most noticeable inhibition (42.58%) of the corneal neovascularization in rats in comparison to the sham treated group, and also exhibited most noticeable inhibition (31.31%) of the oxygen induced retinal neovascularization in rat pups in comparison to the hyperoxia group that was observed for considerable retinal neovascularization.

CONCLUSIONS

The significant antiangiogenic activity evinced by the extract of Telescopium telescopium merits further investigation for ocular neovascular diseases.

摘要

目的

评估来自印度东南沿海的22种软体动物门海洋无脊椎动物的抗血管生成潜力。

方法

收集软体动物物种的活体标本，使用鸡胚绒毛尿囊膜试验评估其甲醇提取物的初步抗血管生成活性。进一步使用大鼠化学烧灼诱导角膜新生血管形成试验和幼鼠氧诱导视网膜病变试验评估提取物的体内抗血管生成活性。

结果

在鸡胚绒毛尿囊膜试验中，四种海洋软体动物物种的甲醇提取物，即文蛤、丽文蛤、长鼻螺和袋蛤甲醇提取物，在200μg的测试浓度下表现出显著的抗血管生成活性，从而显著抑制了血管内皮生长因子（VEGF）诱导的新血管增殖。在这四种提取物中，丽文蛤甲醇提取物表现出相对较高程度的抗血管生成活性，对VEGF诱导的新生血管形成的抑制率为64.63%，其次是长鼻螺甲醇提取物（62.02%）、袋蛤甲醇提取物（60.48%）和文蛤甲醇提取物（47.01%）。对这四种甲醇提取物进一步评估其体内抗血管生成活性，结果显示，与假手术治疗组相比，长鼻螺甲醇提取物对大鼠角膜新生血管形成的抑制作用最为显著（42.58%），与观察到明显视网膜新生血管形成的高氧组相比，对幼鼠氧诱导的视网膜新生血管形成的抑制作用也最为显著（31.31%）。