[鼻息肉型慢性鼻-鼻窦炎中VEGF的表达及克拉霉素对其的调控]
[The expression of VEGF and the regulation of clarithromycin on it in chronic rhinosinusitis with nasal polys].
作者信息
Hu Shuang, You Xuejun, Chen Cuicui, Liu Zheng, Cui Yonghua
出版信息
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2014 Mar;28(5):303-5.
OBJECTIVE
To investigate the expression of vascular endothelial growth factor in the nasal mucosa of chronic rhinosinusitis with nasal polys patients, and explored the regulation of clarithromycin on VEGF.
METHOD
RT-PCR was used to detect the expression of VEGF in nasal mucosa from healthy control and CRSwNP. Nasal mucosal tissue explant culture measure and ELISA were used to explore the effect of clarithromycin on VEGF expression.
RESULT
(1) VEGF mRNA expression level was significantly increased in CRSwNP compared with control and showed a statistic difference (P < 0.01). (2) There was a significant decrease in CRSwNP group undergo clarithromycin treatment on protein expression level of VEGF and showed a statistic difference (P < 0.05).
CONCLUSION
VEGF were overexpressed in CRSwNP group, which presume that play an important role in the pathogenesis of nasal polyps. Clarithromycin may play a therapeutical role on chronic rhinosinusitis through down-regulated the expression of VEGF.
目的
探讨血管内皮生长因子在慢性鼻窦炎伴鼻息肉患者鼻黏膜中的表达情况，并研究克拉霉素对血管内皮生长因子（VEGF）的调控作用。
方法
采用逆转录聚合酶链反应（RT-PCR）检测健康对照者和慢性鼻窦炎伴鼻息肉患者鼻黏膜中VEGF的表达。采用鼻黏膜组织外植体培养法及酶联免疫吸附测定（ELISA）法研究克拉霉素对VEGF表达的影响。
结果
（1）与对照组相比，慢性鼻窦炎伴鼻息肉患者鼻黏膜中VEGF mRNA表达水平显著升高，差异有统计学意义（P < 0.01）。（2）克拉霉素治疗后的慢性鼻窦炎伴鼻息肉患者组VEGF蛋白表达水平显著降低，差异有统计学意义（P < 0.05）。
结论
慢性鼻窦炎伴鼻息肉患者组中VEGF呈过度表达，推测其在鼻息肉发病机制中起重要作用。克拉霉素可能通过下调VEGF的表达对慢性鼻窦炎发挥治疗作用。
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