Zhang X-H, Lu X, Long X-B, You X-J, Gao Q-X, Cui Y-H, Liu Z
Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Clin Exp Allergy. 2009 May;39(5):647-54. doi: 10.1111/j.1365-2222.2008.03198.x. Epub 2009 Mar 2.
Chronic rhinosinusitis without nasal polyps (CRSsNP) and with nasal polyps (CRSwNP) is characterized by persistent inflammation of sinonasal mucosa. Glucocorticoid-induced leucine zipper (GILZ) is a recently described anti-inflammatory mediator.
Here we analysed the expression of GILZ in CRSsNP and CRSwNP, its association with response to surgery, and its cytokine-driven expression regulation in the upper airways. Methods The messenger RNA (mRNA) and protein expression of GILZ in 33 CRSsNP, 32 CRSwNP, and 11 control samples was assessed by means of a quantitative RT-PCR and immunohistochemistry, respectively. Nasal explant culture was used to investigate the effect of IFN-gamma, IL-4, IL-13, IL-1beta, and TNF-alpha on GILZ mRNA expression in normal sinonasal mucosa.
The GILZ mRNA and protein expression was significantly suppressed in both CRSsNP and CRSwNP patients compared with controls. No significant difference in GILZ expression was found between CRSsNP and CRSwNP patients. Comparing patients responsive and patients recalcitrant to surgery, a significant further decrease of GILZ expression was found in recalcitrant patients both in the CRSsNP and in the CRSwNP group. IL-1beta, TNF-alpha, IL-4, and IL-13 reduced, whereas IFN-gamma enhanced GILZ mRNA levels in the sinonasal mucosa.
Down-regulated expression of GILZ may contribute to the pathogenesis of CRSsNP and CRSwNP and associate with response to surgery. GILZ expression in the upper airways can be regulated differentially by different cytokines.
无鼻息肉慢性鼻-鼻窦炎（CRSsNP）和有鼻息肉慢性鼻-鼻窦炎（CRSwNP）的特征是鼻窦黏膜持续炎症。糖皮质激素诱导亮氨酸拉链蛋白（GILZ）是最近描述的一种抗炎介质。
在此，我们分析了GILZ在CRSsNP和CRSwNP中的表达、其与手术反应的关联以及其在上呼吸道中细胞因子驱动的表达调控。方法分别通过定量逆转录-聚合酶链反应（RT-PCR）和免疫组化评估33例CRSsNP、32例CRSwNP和11例对照样本中GILZ的信使核糖核酸（mRNA）和蛋白表达。采用鼻外植体培养研究干扰素-γ（IFN-γ）、白细胞介素-4（IL-4）、白细胞介素-13（IL-13）、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）对正常鼻窦黏膜中GILZ mRNA表达的影响。
与对照组相比，CRSsNP和CRSwNP患者的GILZ mRNA和蛋白表达均显著受抑制。CRSsNP和CRSwNP患者之间的GILZ表达无显著差异。比较手术有反应和无反应的患者，在CRSsNP和CRSwNP组中，无反应患者的GILZ表达均进一步显著降低。IL-1β、TNF-α、IL-4和IL-13降低了鼻窦黏膜中GILZ mRNA水平，而IFN-γ增强了该水平。
GILZ表达下调可能参与CRSsNP和CRSwNP的发病机制，并与手术反应相关。上呼吸道中GILZ的表达可由不同细胞因子进行差异调节。
