Uribe-Gutiérrez Gabriel, Hernández-Santos Héctor, Manjarrez-Zavala María Eugenia, Rosete-Olvera Dora Patricia, Nava-Frías Margarita, Moreno-Espinosa Sarbelio, Velázquez-Guadarrama Norma, Gómez Rocío, González-Márquez Humberto, Fierro Reyna, Mejía-Arangure Juan Manuel, Zavala-Vega Sergio, Hernández-Fernández Modesto, Arellano-Galindo José
Clin Lab. 2014;60(8):1277-85. doi: 10.7754/clin.lab.2013.130449.
Adenovirus (AdV) causes respiratory infection; recent observations suggest that some subtypes have more ability to develop fatal disease. AdV infection has been associated with co-infection with human bocavirus (HBoV). We analysed the frequency of AdV infection, its subtypes and the presence of co-infection with HBoV, as well the clinical characteristics of such co-infection in Mexican paediatric immunosuppressed (IP) and non-immunosuppressed patients (non-IP) diagnosed with pneumonia.
A total of 5185 nasopharyngeal swabs from two groups of children with pneumonia, one IP and the other non-IP, were analysed for the detection of AdV by immunofluorescence and confirmed by PCR and culture. HBoV was identified by PCR. Positive samples for AdV and AdV/HBoV were typed using PCR sequencing, the clinical characteristics of the AdV/HBoV co-infection were analysed.
Thirty-seven of the 5185 (0.71%) samples were positive for AdV, of those 27/37 (73%) were detected in non-IP and 10/37 (27%) in the IP group. Twelve were typed as follows: 9/12 (75%) as Species B1 subtype 3, of those 8/9 (88.9%) in non-IP and 1/9 in the IP group. One of twelve AdV2 subtype B11a was identified in one non-IP and the remaining two out of 12 successfully typed, were identified as Species C subtypes 2 and 6 in the group of non-IP. The presence of both AdV and HBoV1 in co-infection was observed in 2/37 (5.4%) non-IP with a syndrome like influenza.
In this 5 year analysis of samples from non-IP and IP hospitalized paediatric patients with a diagnosis of pneumonia, a low incidence of AdV was found. B1 was the most frequent subtype and frequently found in non-IP, and two cases of co-infection AdV/HBoV1 were detected in two non-IP with a influenza-like syndromes. This is the first report of HBoV and AdV co-infection in Mexico. The frequency of AdV and HBoV co-infection was lower than that reported in other populations.
腺病毒（AdV）可引起呼吸道感染；最近的观察表明，某些亚型引发致命疾病的能力更强。腺病毒感染与人类博卡病毒（HBoV）的合并感染有关。我们分析了墨西哥诊断为肺炎的儿科免疫抑制（IP）和非免疫抑制患者（非IP）中腺病毒感染的频率、其亚型以及与人类博卡病毒合并感染的情况，以及这种合并感染的临床特征。
对两组肺炎儿童（一组为免疫抑制组，另一组为非免疫抑制组）共5185份鼻咽拭子进行分析，通过免疫荧光检测腺病毒，并通过聚合酶链反应（PCR）和培养进行确认。通过PCR鉴定人类博卡病毒。对腺病毒和腺病毒/人类博卡病毒阳性样本进行PCR测序分型，分析腺病毒/人类博卡病毒合并感染的临床特征。
5185份样本中有37份（0.71%）腺病毒呈阳性，其中27/37（73%）在非免疫抑制组中检测到，10/37（27%）在免疫抑制组中检测到。12份样本分型如下：9/12（75%）为B1种3型，其中8/9（88.9%）在非免疫抑制组，1/9在免疫抑制组。在一名非免疫抑制患者中鉴定出12份腺病毒2型中的1份为B11a亚型，其余12份中成功分型的另外2份，在非免疫抑制组中鉴定为C种2型和6型。在2/37（5.4%）非免疫抑制患者中观察到腺病毒和人类博卡病毒1型合并感染，伴有类似流感的综合征。
在对诊断为肺炎的住院儿科非免疫抑制和免疫抑制患者的样本进行的这5年分析中，发现腺病毒感染率较低。B1是最常见的亚型，且常见于非免疫抑制患者中，在两名患有流感样综合征的非免疫抑制患者中检测到两例腺病毒/人类博卡病毒1型合并感染。这是墨西哥关于人类博卡病毒和腺病毒合并感染的首次报告。腺病毒和人类博卡病毒合并感染的频率低于其他人群的报告频率。
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