Department of Microbiology, Jahangirnagar University, Dhaka, Bangladesh.
Department of Physiology, Pabna Medical College, Pabna, Bangladesh.
J Med Virol. 2020 Dec;92(12):3194-3201. doi: 10.1002/jmv.25812. Epub 2020 Apr 8.
Virus associated diarrhea remains one of the leading causes of children morbidity and mortality in Bangladesh. Human bocavirus (HBoV) has been reported as a potential pathogen of children's diarrhea worldwide. However, due to its frequent association with other gastroenteric pathogens, its role as diarrhea causative agent remains to be defined. This study focuses to detect the incidence of HBoV and adenovirus (AdV) and to determine the molecular and epidemiological characteristics of HBoV and AdV. Between January 2015 to January 2019, 290 fecal specimens were collected from diarrheal children in Bangladesh. All fecal specimens were tested for HBoV and AdV by conventional polymerase chain reaction and sequencing methods. HBoV was detected in 7.24% (21 of 290) of the stool samples, as a sole virus in 71.42% (15 of 21) of the positive samples. AdV was detected in 4.82% (14 of 290) of the samples. The most common clinical symptoms of HBoV infected patients were diarrhea (100%) and vomiting (57%). All of the isolates of HBoV were from HBoV1 and AdV were from AdV41, AdV5, AdV7, and AdV8. To the best of our knowledge, this is the first epidemiological and molecular analysis report of HBoV from clinical specimens in Bangladesh. In the future, more studies are needed to clarify the role of HBoV as diarrheal pathogens.
病毒相关性腹泻仍然是孟加拉国儿童发病率和死亡率的主要原因之一。人类博卡病毒（HBoV）已被报道为全球儿童腹泻的潜在病原体。然而，由于其经常与其他肠道病原体相关，其作为腹泻病原体的作用仍有待确定。本研究旨在检测 HBoV 和腺病毒（AdV）的发病率，并确定 HBoV 和 AdV 的分子和流行病学特征。2015 年 1 月至 2019 年 1 月，从孟加拉国腹泻儿童中采集了 290 份粪便标本。所有粪便标本均采用常规聚合酶链反应和测序方法检测 HBoV 和 AdV。在 290 份粪便样本中，有 7.24%（21 份）检测到 HBoV，在 21 份阳性样本中，有 71.42%（15 份）为单一病毒。在 290 份样本中，有 4.82%（14 份）检测到 AdV。HBoV 感染患者最常见的临床症状是腹泻（100%）和呕吐（57%）。所有 HBoV 分离株均来自 HBoV1，AdV 来自 AdV41、AdV5、AdV7 和 AdV8。据我们所知，这是孟加拉国首次从临床标本中对 HBoV 进行的流行病学和分子分析报告。未来需要进一步研究来阐明 HBoV 作为腹泻病原体的作用。
