完全缺失淋巴细胞和自然杀伤细胞的裸鼠的建立及其在体内生物成像中的应用。
Establishment of nude mice with complete loss of lymphocytes and NK cells and application for in vivo bio-imaging.
作者信息
Kariya Ryusho, Matsuda Kouki, Gotoh Kumiko, Vaeteewoottacharn Kulthida, Hattori Shinichiro, Okada Seiji
机构信息
Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, Kumamoto, Japan.
Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, Kumamoto, Japan
出版信息
In Vivo. 2014 Sep-Oct;28(5):779-84.
BACKGROUND
Nude mice are used in human xenograft research; however, only 25-35% of human tumors have been successfully transplanted into nude mice and their application is limited due to high natural killer (NK) cell activity. More severely immunodeficient mice with loss of NK activity are needed to overcome this limitation.
MATERIALS AND METHODS
Balb/c nude Rag-2(-/-)Jak3(-/-) (Nude-RJ) mice were established by crossing Rag-2(-/-)Jak3(-/-) mice and nude mice. The K562 cell line was implanted subcutaneously to compare tumorigenicity between Nude-RJ mice and Nude mice. The cholangiocarcinoma mCherry expressing cell line (KKU-M213) was implanted subcutaneously, and fluorescence intensity and tumor weight were measured.
RESULTS
Nude R/J mice showed complete loss of lymphocytes and NK cells. Xeno-transplantation of K562 cells showed higher proliferation in Nude R/J mice than nude mice. Subcutaneously-transplanted mCherry-transduced KKU-M213 cells were successfully detected with a fluorescence imager.
CONCLUSION
Nude-R/J mice are valuable tools for in vivo imaging studies in biomedical research.
背景
裸鼠用于人类异种移植研究；然而，只有25% - 35%的人类肿瘤能成功移植到裸鼠体内，且由于自然杀伤（NK）细胞活性高，其应用受到限制。需要更严重免疫缺陷且NK活性丧失的小鼠来克服这一限制。
材料与方法
通过将Rag-2(-/-)Jak3(-/-)小鼠与裸鼠杂交建立Balb/c裸Rag-2(-/-)Jak3(-/-)（裸-RJ）小鼠。皮下植入K562细胞系，比较裸-RJ小鼠和裸鼠之间的致瘤性。皮下植入表达胆管癌mCherry的细胞系（KKU-M213），并测量荧光强度和肿瘤重量。
结果
裸R/J小鼠表现出淋巴细胞和NK细胞完全缺失。K562细胞的异种移植在裸R/J小鼠中比在裸鼠中增殖更高。皮下移植的mCherry转导的KKU-M213细胞用荧光成像仪成功检测到。
结论
裸-R/J小鼠是生物医学研究中体内成像研究的有价值工具。
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