Garcia-Contreras Rene, Scougall-Vilchis Rogelio J, Contreras-Bulnes Rosalia, Kanda Yumiko, Nakajima Hiroshi, Sakagami Hiroshi
Dental and Advanced Studies Research Center, Faculty of Dentistry, Autonomous University State of Mexico, Toluca, Mexico Division of Pharmacology, Meikai University School of Dentistry, Sakado, Saitama, Japan
Department of Orthodontics, Faculty of Dentistry, Autonomous University State of Mexico, Toluca, Mexico.
In Vivo. 2014 Sep-Oct;28(5):895-907.
BACKGROUND/AIM: Incorporation of nanoparticles (NPs) into the glass ionomer cements (GICs) is known to improve their mechanical and antibacterial properties. The present study aimed to investigate the possible cytotoxicity and pro-inflammation effect of three different powdered GICs (base, core build and restorative) prepared with and without titanium dioxide (TiO2) nanoparticles.
Each GIC was blended with TiO2 nanopowder, anatase phase, particle size <25 nm at 3% and 5% (w/w), and the GIC blocks of cements were prepared in a metal mold. The GICs/TiO2 nanoparticles cements were smashed up with a mortar and pestle to a fine powder, and then subjected to the sterilization by autoclaving. Human oral squamous cell carcinoma cell lines (HCS-2, HSC-3, HSC-4, Ca9-22) and human normal oral cells [gingival fibroblast (HGF), pulp (HPC) and periodontal ligament fibroblast (HPLF)] were incubated with different concentrations of GICs in the presence or absence of TiO2 nanoparticles, and the viable cell number was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Prostaglandin E2 was quantified by enzyme-linked immunosorbent assay (ELISA). Changes in fine cell structure were assessed by transmission electron microscopy.
Cancer cells exhibited moderate cytotoxicity after 48 h of incubation, regardless of the type of GIC and the presence or absence of TiO2 NPs. GICs induced much lower cytotoxicity against normal cells, but induced prostaglandin E2 production, in a synergistic wanner with interleukin-1β.
The present study shows acceptable to moderate biocompatibility of GICs impregnated with TiO2 nanoparticles, as well as its pro-inflammatory effects at higher concentrations.
背景/目的：已知将纳米颗粒（NPs）掺入玻璃离子水门汀（GICs）可改善其机械性能和抗菌性能。本研究旨在调查三种不同的粉状GICs（基底、核芯构建和修复型）在添加和不添加二氧化钛（TiO₂）纳米颗粒的情况下可能产生的细胞毒性和促炎作用。
每种GIC与粒径<25 nm的锐钛矿相TiO₂纳米粉末以3%和5%（w/w）的比例混合，并在金属模具中制备GIC块体。将GICs/TiO₂纳米颗粒水门汀用研钵和研杵捣碎成细粉，然后通过高压灭菌进行消毒。将人口腔鳞状细胞癌细胞系（HCS-2、HSC-3、HSC-4、Ca9-22）和人正常口腔细胞[牙龈成纤维细胞（HGF）、牙髓（HPC）和牙周膜成纤维细胞（HPLF）]在有或无TiO₂纳米颗粒的情况下与不同浓度的GICs一起孵育，并通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法测定活细胞数。通过酶联免疫吸附测定（ELISA）定量前列腺素E₂。通过透射电子显微镜评估细胞细微结构的变化。
孵育48小时后，癌细胞表现出中度细胞毒性，无论GIC的类型以及是否存在TiO₂ NPs。GICs对正常细胞诱导的细胞毒性要低得多，但会诱导前列腺素E₂的产生，与白细胞介素-1β协同作用。
本研究表明，浸渍有TiO₂纳米颗粒的GICs具有可接受至中度的生物相容性，以及在较高浓度下的促炎作用。
