Discipline of Prosthodontics, Operative Dentistry and Endodontics, Faculty of Dentistry, National University of Singapore, Singapore.
Discipline of Oral Sciences, Faculty of Dentistry, National University of Singapore, Singapore, 119083, Singapore.
J Esthet Restor Dent. 2017 Apr;29(2):146-156. doi: 10.1111/jerd.12282. Epub 2017 Feb 12.
We are introducing novel glass-ionomer cement (GIC) dually-modified with chitosan (CH) in the liquid phase and titanium-dioxide nano-powder (TiO /NP) in the powder phase. The aim was to investigate the effect of this dual-modification on the antibacterial properties against S. mutans biofilms and on the bulk and surface mechanical properties.
Commercially available powder/liquid restorative GIC was used in this study. The GIC specimens were modified with 3% (w/w) TiO /NP, 10% (v/v) CH solution, or dually-modified with TiO /CH. The non-modified GIC was used as a control. The biofilms formations were characterized by SEM, live/dead assay using confocal-microscopy, colony-forming unit counts, and MTS assay. The bulk and surface mechanical properties were characterized in terms of flexural and compressive strengths and surface hardness, respectively.
With the dual-modification, a significant improvement in the antibacterial properties was found both qualitatively and quantitatively. The synergetic effect of the dual-modification was also reflected on the enhancement of the flexural and compressive strengths. However, no difference was found in surface hardness.
The modification of GIC powder with TiO /NP showed to be more effective in enhancing the mechanical properties. However, the enhancement in the antibacterial properties was more evident with CH incorporation in GIC liquid.
This study introduced novel glass ionomer cement dually-modified with TiO2 NP and chitosan with superior mechanical and antibacterial properties for potential applications in restorative and preventive dentistry. The modification of GIC powder with TiO2 NP showed to be more effective in enhancing the mechanical properties. However, the enhancement in the antibacterial properties was more evident with CH incorporation in GIC liquid. Although of the promising synergetic effect of the dual-modification of GIC with TiO2 NP/CH, further clinically-related studies are recommended. (J Esthet Restor Dent 29:146-156, 2017).
我们介绍了一种新型的玻璃离子水门汀（GIC），它在液相中用壳聚糖（CH）进行双重改性，在粉末相中用二氧化钛纳米粉末（TiO 2 /NP）进行双重改性。目的是研究这种双重改性对抑制变形链球菌生物膜的抗菌性能以及对块状和表面力学性能的影响。
本研究采用市售的粉液型修复性 GIC。GIC 试件用 3%（w/w）TiO 2 /NP、10%（v/v）CH 溶液或 TiO 2 /CH 双重改性。未改性的 GIC 用作对照。采用扫描电子显微镜（SEM）、共聚焦显微镜活/死检测、菌落形成单位计数和 MTS 检测对生物膜形成进行了表征。分别采用弯曲强度和压缩强度以及表面硬度来表征块状和表面力学性能。
采用双重改性，在定性和定量两方面均发现抗菌性能显著提高。双重改性的协同作用也反映在增强弯曲强度和压缩强度上。然而，表面硬度没有差异。
TiO 2 /NP 对 GIC 粉末的改性在增强机械性能方面更为有效。然而，在 GIC 液体中加入 CH 可更明显地提高抗菌性能。
本研究介绍了一种新型的玻璃离子水门汀，它用 TiO 2 NP 和壳聚糖双重改性，具有优异的机械性能和抗菌性能，有望应用于修复和预防牙科领域。TiO 2 /NP 对 GIC 粉末的改性在增强机械性能方面更为有效。然而，在 GIC 液体中加入 CH 可更明显地提高抗菌性能。虽然 TiO 2 NP/CH 双重改性的协同作用有很大的潜力，但建议进行进一步的临床相关研究。（J Esthet Restor Dent 29:146-156，2017）。
