Wang Yuehua, Feng Xianmin, Jiang Linzhe
Department of Pathogenic Biology, Jilin Medical College, Jilin, Jilin 132013, China. Email:
Department of Pathogenic Biology, Jilin Medical College, Jilin, Jilin 132013, China.
Chin Med J (Engl). 2014;127(17):3165-70.
The objective of this article was to review the current advances in diagnostic methods for Acanthamoeba keratitis (AK).
Data used in this review were retrieved from PubMed (1970-2013). The terms "Acanthamoeba keratitis" and "diagnosis" were used for the literature search.
Data from published articles regarding AK and diagnosis in clinical trials were identified and reviewed.
The diagnostic methods for the eight species implicated in AK were reviewed. Among all diagnostic procedures, corneal scraping and smear examination was an essential diagnostic method. Polymerase chain reaction was the most sensitive and accurate detection method. Culturing of Acanthamoeba was a reliable method for final diagnosis of AK. Confocal microscopy to detect Acanthamoeba was also effective, without any invasive procedure, and was helpful in the early diagnosis of AK.
Clinically, conjunction of various diagnostic methods to diagnose AK was necessary.
本文旨在综述棘阿米巴角膜炎（AK）诊断方法的当前进展。
本综述所用数据取自PubMed（1970 - 2013年）。检索词为“棘阿米巴角膜炎”和“诊断”。
确定并综述已发表文章中有关AK及临床试验诊断的数据。
对涉及AK的8个物种的诊断方法进行了综述。在所有诊断程序中，角膜刮片和涂片检查是一种基本的诊断方法。聚合酶链反应是最敏感和准确的检测方法。棘阿米巴培养是AK最终诊断的可靠方法。共聚焦显微镜检测棘阿米巴也有效，无需任何侵入性操作，有助于AK的早期诊断。
临床上，联合多种诊断方法诊断AK是必要的。
Zotero 插件