Wang Meixu, Sun Guangxu, Sun Yangkai, You Xiaomin, Li Xiaoxue, Cheng Yang, Xuan Yinghua
Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, 214122, P.R. China.
Public Health Research Center, Jiangnan University, Wuxi, Jiangsu, 214122, P.R. China.
Korean J Parasitol. 2018 Dec;56(6):615-618. doi: 10.3347/kjp.2018.56.6.615. Epub 2018 Dec 31.
Members of genus Acanthamoeba are widely distributed in the environment. Some are pathogenic and cause keratitis and fatal granulomatous amoebic encephalitis. In this study, we isolated an Acanthamoeba CJW/W1 strain from tap water in Wuxi, Jiangsu Province, China. Its 18S rDNA was sequenced and a phylogenetic tree was constructed. The isolated cysts belonged to morphologic group II. Comparison of 18S rDNA sequences of CJW/W1 strain and other isolates showed high similarity (99.7%) to a clinical isolate Asp, KA/E28. A phylogeny analysis confirmed this isolate belonged to the pathogenic genotype T4, the most common strain associated with Acanthamoeba-related diseases. This is the first report of an Acanthamoeba strain isolated from tap water in Wuxi, China. Acanthamoeba could be a public health threat to the contact lens wearers and, therefore, its prevalence should be monitored.
棘阿米巴属的成员广泛分布于环境中。其中一些具有致病性，可导致角膜炎和致命的肉芽肿性阿米巴脑炎。在本研究中，我们从中国江苏省无锡市的自来水中分离出一株棘阿米巴CJW/W1菌株。对其18S rDNA进行测序并构建了系统发育树。分离出的包囊属于形态学第II组。CJW/W1菌株与其他分离株的18S rDNA序列比较显示，与临床分离株Asp、KA/E28具有高度相似性（99.7%）。系统发育分析证实该分离株属于致病基因型T4，这是与棘阿米巴相关疾病最常见的菌株。这是中国无锡自来水中分离出棘阿米巴菌株的首次报道。棘阿米巴可能对隐形眼镜佩戴者构成公共卫生威胁，因此，应监测其流行情况。
