Xing Na, Liang Yajun, Gao Zhenzhen, He Junping, He Xiaoyan, Li Hongquan, Dong Changsheng
Institute of Animal Biotechnology, College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China.
Institute of Animal Biotechnology, College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China.
Acta Histochem. 2014 Oct;116(8):1301-6. doi: 10.1016/j.acthis.2014.07.014. Epub 2014 Sep 2.
The objective of the present study was to investigate the temporal and spatial expression of Smad2 and Smad4 proteins, the downstream signaling molecules of the transforming growth factor beta (TGF-β) superfamily, in the porcine ovary. Cellular localization of Smad2 and Smad4 proteins was examined using immunohistochemistry. The specificity of the antibodies was examined using Western blot assay. Western blot analyses demonstrated that 52kDa Smad2 and 60kDa Smad4 proteins were expressed in the porcine ovary. Immunohistochemistry revealed that Smad2 and Smad4 were widely expressed in the porcine ovary, mainly localized in the oocyte, granulosa and thecal cells at different stages of folliculogenesis. Within the primordial and primary follicles, Smad2 and Smad4 showed strong staining in oocytes and follicular cells. In the antral follicle, strong staining was observed in oocytes, granulosa and theca cells. These findings suggest that Smad2 and Smad4 may be a key regulator of follicular development and growth of oocytes in the porcine ovary.
本研究的目的是调查转化生长因子β（TGF-β）超家族的下游信号分子Smad2和Smad4蛋白在猪卵巢中的时空表达。采用免疫组织化学方法检测Smad2和Smad4蛋白的细胞定位。使用蛋白质免疫印迹法检测抗体的特异性。蛋白质免疫印迹分析表明，52kDa的Smad2和60kDa的Smad4蛋白在猪卵巢中表达。免疫组织化学显示，Smad2和Smad4在猪卵巢中广泛表达，主要定位于卵泡发生不同阶段的卵母细胞、颗粒细胞和卵泡膜细胞。在原始卵泡和初级卵泡内，Smad2和Smad4在卵母细胞和卵泡细胞中呈强染色。在窦状卵泡中，卵母细胞、颗粒细胞和卵泡膜细胞中观察到强染色。这些发现表明，Smad2和Smad4可能是猪卵巢中卵泡发育和卵母细胞生长的关键调节因子。
