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PTSMAD4在三疣梭子蟹精子发生中的功能研究

Functional Study of PTSMAD4 in the Spermatogenesis of the Swimming Crab .

作者信息

Zhang Yu, Xiang Qiu-Meng, Mu Chang-Kao, Wang Chun-Lin, Hou Cong-Cong

机构信息

Key Laboratory of Aquacultural Biotechnology, Key Laboratory of Marine Biotechnology of Zhejiang Province, College of Marine Sciences, Ningbo University, Ningbo 315211, China.

出版信息

Int J Mol Sci. 2024 Dec 6;25(23):13126. doi: 10.3390/ijms252313126.

DOI:10.3390/ijms252313126
PMID:39684836
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11642582/
Abstract

holds significant economic value. The spermatogenesis is regulated by numerous signaling pathways. Among them, the TGF-β signaling pathway plays an important role in the development of testes and spermatogenesis. Smad4 is a Co-Smad protein that forms a complex with R-Smad to regulate the expression of target genes. The sperm structure in crustaceans differs greatly from that in mammals, with mature sperm lacking tails. Our previous studies have reported the function of R-Smad in the spermatogenesis of . In this study, we cloned the full-length cDNA sequence of ; immunofluorescence technology revealed that PTSMAD4 is expressed throughout all stages of spermatogenesis. We knocked down the expression of PTSMAD4 in using RNAi technology, and the immunofluorescence results show abnormal co-localization and weakened signals of PTSMAD4 and PTSMAD2. Additionally, transcriptome sequencing results enriched functional genes and pathways related to spermatogenesis. This study indicates that PTSMAD4 may participate in the spermatogenesis process through its involvement in signal transduction. This research not only lays the foundation for further study of the function of the TGF-β signaling pathway in spermatogenesis but also provides a theoretical basis for further investigation of the spermatogenesis mechanism in crustaceans.

摘要

具有重要的经济价值。精子发生受众多信号通路调控。其中，转化生长因子-β（TGF-β）信号通路在睾丸发育和精子发生中起重要作用。Smad4是一种共Smad蛋白，它与受体调节型Smad（R-Smad）形成复合物以调节靶基因的表达。甲壳类动物的精子结构与哺乳动物的有很大差异，成熟精子没有尾巴。我们之前的研究报道了R-Smad在[具体物种]精子发生中的功能。在本研究中，我们克隆了[具体基因]的全长cDNA序列；免疫荧光技术显示PTSMAD4在精子发生的所有阶段均有表达。我们利用RNA干扰技术敲低了[具体物种]中PTSMAD4的表达，免疫荧光结果显示PTSMAD4和PTSMAD2的共定位异常且信号减弱。此外，转录组测序结果富集了与精子发生相关的功能基因和通路。本研究表明PTSMAD4可能通过参与信号转导而参与精子发生过程。本研究不仅为进一步研究TGF-β信号通路在精子发生中的功能奠定了基础，也为进一步探究甲壳类动物精子发生机制提供了理论依据。

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