Luo Lei, Zhu Runan, Zhao Linqing, Deng Jie, Wang Fang, Sun Yu, Song Qinwei, Ding Yaxin, Qian Yuan
Laboratory of Virology, Capital Institute of Pediatrics, Beijing 100020, China.
Laboratory of Virology, Capital Institute of Pediatrics, Beijing 100020, China. Email:
Zhonghua Er Ke Za Zhi. 2014 Jun;52(6):444-8.
Human parechovirus (HPeV) is a single-stranded, positive sense RNA virus in the Parechovirus genus within the large family of Picornaviridae. As a possible new pathogen of neonatal sepsis, meningoencephalitis and other infections in young children, HPeV gets more and more attention. This study aimed to better understand the association of HPeV with central nervous system (CNS) infectious diseases and sepsis among hospitalized children in Beijing.
A total of 577 cerebrospinal fluid (CSF) samples were retrospectively collected from 557 children suspected of CNS infections in 2012. Three hundred and fifty-one of them were male and 206 were female. HPeV was screened by reverse transcription-nested PCR (RT-nPCR) with the universal primers which target the highly conserved 5'UTR. The positive samples were genotyped by amplifying and sequencing for the VP3/VP1 junction region. The sequences were compared with the HPeV sequences from GenBank and performed phylogenetic analysis.Some samples other than CSF from HPeV positive children, including serum, nasopharyngeal aspirate and stool, were collected and carried out screening for HPeV.
With the RT-nPCR by universal primers, HPeVs were detected in 18 out of 577 CSF samples obtained from 18 children with a positive rate of 3.1%. The ratio of male and female was 2: 1. There were no statistically significant differences on infection rate between boys (12/351, 3.4%) and girls (6/206, 2.9%). All of 18 positive CSF samples were negative for enterovirus, Epstein-Barr virus (EBV), human cytomegalovirus (HCMV), and herpes simplex virus 1 and 2 (HSV).HPeVs from 10 positive CSF samples were genotyped successfully, consisting of 7 HPeV3 and 3 HPeV1. In addition, 2 of 8 serum samples were positive for HPeV3 and 1 of 2 stool samples were positive for HPeV 1. HPeVs were identified in CSF from children aged from 15 days to 14 years, in which 7 cases were infants younger than 3 months and 5 cases were infants from 3 months to one year. Three children older than the age of 9 years (9, 13 and 14 years) were positive for HPeV. Most of the children (6/8) infected with HPeV3 were younger than 3 months and were diagnosed as sepsis, while the rest of HPeV3 positive children were diagnosed as meningitis and bronchopneumonia. HPeV3 infection clustered in August, while HPeV1 in January.
HPeVs were associated with CNS infections and sepsis in hospitalized children in Beijing, especially in children younger than one year.HPeV3 was the predominant type identified in CSF.
人微小病毒（HPeV）是微小核糖核酸病毒科中微小病毒属的一种单链正义RNA病毒。作为新生儿败血症、脑膜脑炎及其他幼儿感染可能的新病原体，HPeV受到越来越多的关注。本研究旨在更好地了解北京住院儿童中HPeV与中枢神经系统（CNS）传染病及败血症的关联。
回顾性收集2012年557例疑似CNS感染儿童的577份脑脊液（CSF）样本。其中男性351例，女性206例。采用针对高度保守的5'UTR的通用引物，通过逆转录巢式PCR（RT-nPCR）筛选HPeV。对阳性样本的VP3/VP1连接区进行扩增和测序以进行基因分型。将序列与GenBank中的HPeV序列进行比较并进行系统发育分析。收集HPeV阳性儿童除CSF外的其他一些样本，包括血清、鼻咽抽吸物和粪便，并进行HPeV筛查。
使用通用引物进行RT-nPCR，在577份CSF样本中的18份中检测到HPeV，来自18名儿童，阳性率为3.1%。男女比例为2:1。男孩（12/351，3.4%）和女孩（6/206，2.9%）的感染率无统计学差异。18份阳性CSF样本中肠道病毒、EB病毒（EBV）、人巨细胞病毒（HCMV）以及单纯疱疹病毒1型和2型（HSV）均为阴性。10份阳性CSF样本中的HPeV成功进行了基因分型，包括7株HPeV3和3株HPeV1。此外，8份血清样本中有2份HPeV3阳性，2份粪便样本中有1份HPeV1阳性。在年龄从15天至14岁儿童的CSF中检测到HPeV，其中7例为3个月以下婴儿，5例为3个月至1岁婴儿。9岁以上（9、13和14岁）的3名儿童HPeV阳性。大多数感染HPeV3的儿童（6/8）年龄小于3个月，被诊断为败血症，其余HPeV3阳性儿童被诊断为脑膜炎和支气管肺炎。HPeV3感染集中在8月，而HPeV1感染集中在次年1月。
HPeV与北京住院儿童的CNS感染及败血症相关，尤其是1岁以下儿童。HPeV3是CSF中鉴定出的主要类型。
